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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200801099
Degtyarev et al.
• Figure S1 Relative levels of Akt isoforms in cancer cell lines and inducible KD of Akt isoforms in U87MG cells and xenograft tumors.
• Figure S2 Effect of GFP-targeting shRNA and Akt isoform KDs on cell cycle progression, apoptosis, and autophagy.
• Figure S3 Effect of LAMP2, Atg7, protease inhibitors, cathepsin D siRNA, and pepstatin A on PC3 cell viability in combination with PI-103 or Akti-1/2.
• Figure S4 CQ promoted mitochondrial membrane depolarization and cellular ROS accumulation in combination with Akti-1/2.
• Figure S5 NAC rescued cell death induced by Akti + CQ.
• Video 1 Time-lapse video of PC3 cells treated with DMSO control under 5% FBS culture condition over a 3-d period at 1-h intervals between frames.
• Video 2 Time-lapse video of PC3 cells treated with 10 µM CQ under 5% FBS culture condition over a 3-d period at 1-h intervals between frames.
• Video 3 Time-lapse video of PC3 cells treated with 10 µM Akti-1/2 under 5% FBS culture condition over a 3-d period at 1-h intervals between frames.
• Video 4 Time-lapse video of PC3 cells treated with 10 µM CQ and 10 µM Akti-1/2 under 5% FBS culture condition over a 3-d period at 1-h intervals between frames.
• Tables S1 & S2 (PDF)
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