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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200801125
Uteng et al.
• Figure S1 Spindle formation in X. laevis egg extract after immunodepletion of Eg5 and the addition of recombinant Eg5 constructs.
• Figure S2 Dynamics of Eg5 and tubulin measured by photoactivation and photobleaching experiments, respectively, and control experiments testing for potential contribution of photobleaching during image acquisition.
• Figure S3 Microtubule flux measured by speckle microscopy.
• Figure S4 Dynein–dynactin localization in unperturbed and cc1- and p50-treated spindles.
• Figure S5 Analysis of functionality of Eg5∆C in vitro and inside spindles.
• Video 1 Time-lapse fluorescence video of photoactivated Eg5-paGFP in a spindle.
• Video 2 Time-lapse fluorescence video of Cy5 microtubules after photobleaching.
• Video 3 Time-lapse fluorescence video showing simultaneously Eg5-paGFP and Cy5 microtubules.
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