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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200806118
Yue et al.

• Figure S1 Phenotype of Survivin^OFF cells and rescue by Survivin-GFP.

• Figure S2 CDK phosphorylation does not appear to regulate Survivin function.

• Figure S3 Aurora B kinase fails to localize to centromeres in early mitosis but localizes normally in anaphase/telophase in cells expressing Survivin^D72A/D73A.

• Figure S4 Further characterization of Survivin linker mutants.

• Video 1 Wild-type DT40 cells in culture.

• Video 2 Cells lacking Survivin fail to complete cytokinesis and subsequently die.

• Video 3 A Survivin^ON DT40 cell expressing wild-type Survivin completes mitosis normally.

• Video 4 A cell lacking Survivin reaches normal metaphase and initiates anaphase, during which sister chromatid separation occurs.

• Video 5 A cell lacking Survivin initiates anaphase, but sister chromatids collapse back together, cytokinesis fails, and a single nucleus forms.

• Video 6 Cells lacking Survivin fail to complete cytokinesis and subsequently die.

• Video 7 Cells lacking Survivin fail to complete cytokinesis and subsequently die during interphase.

 

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