Drosophila Atypical Protein Kinase C Associates with Bazooka and Controls Polarity of Epithelia and Neuroblasts

doi:10.1083/jcb.150.6.1361

16th INTERNATIONAL VASCULAR BIOLOGY MEETING

Published online 18 September 2000. doi:10.1083/jcb.150.6.1361

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© The Rockefeller University Press, 0021-9525/2000//1361 $5.00
The Journal of Cell Biology, Volume 150, Number 6, , 2000 1361-1374

Original Article

Drosophila Atypical Protein Kinase C Associates with Bazooka and Controls Polarity of Epithelia and Neuroblasts

Andreas Wodarz^a, Andreas Ramrath^a, Alexandra Grimm^a, and Elisabeth Knust^a

^a Institut für Genetik, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany
Institut für Genetik, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany.+49-211-8112279+49-211-8114743

The establishment and maintenance of polarity is of fundamentalimportance for the function of epithelial and neuronal cells.In Drosophila, the multi-PDZ domain protein Bazooka (Baz) isrequired for establishment of apico-basal polarity in epitheliaand in neuroblasts, the stem cells of the central nervous system.In the latter, Baz anchors Inscuteable in the apical cytocortex,which is essential for asymmetric localization of cell fatedeterminants and for proper orientation of the mitotic spindle.Here we show that Baz directly binds to the Drosophila atypicalisoform of protein kinase C and that both proteins are mutuallydependent on each other for correct apical localization. Loss-of-functionmutants of the Drosophila atypical isoform of PKC show lossof apico-basal polarity, multilayering of epithelia, mislocalizationof Inscuteable and abnormal spindle orientation in neuroblasts.Together, these data provide strong evidence for the existenceof an evolutionary conserved mechanism that controls apico-basalpolarity in epithelia and neuronal stem cells. This study isthe first functional analysis of an atypical protein kinaseC isoform using a loss-of-function allele in a genetically tractableorganism.

Key Words: cell polarity • atypical PKC • Bazooka • tight junction • asymmetric cell division

Abbreviations used in this paper: Arm, Armadillo; Baz, Bazooka;CNS, central nervous system; Crb, Crumbs; DaPKC, Drosophilaatypical PKC; Insc, Inscuteable; Nrt, Neurotactin; sdt, stardust;TJ, tight junction; ZA, zonula adherens.

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