Molecular requirements for actin-based lamella formation in Drosophila S2 cells

doi:10.1083/jcb.200303023

Published 15 September 2003. doi:10.1083/jcb.200303023

This Article

	Full Text
	Full Text (PDF, 369K)
	PPT slides of all figures
	Supplemental Material Index
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Rogers, S. L.
	Articles by Vale, R. D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Rogers, S. L.
	Articles by Vale, R. D.


Related Collections

	Related Article

Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2003/9/1079 $5.00
The Journal of Cell Biology, Volume 162, Number 6, 1079-1088

Article

Molecular requirements for actin-based lamella formation in Drosophila S2 cells

Stephen L. Rogers, Ursula Wiedemann, Nico Stuurman and Ronald D. Vale

Howard Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94107

Address correspondence to Ron Vale, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, N312 Genentech Hall, 600 16th Street, San Francisco, CA 94107. Tel.: (415) 476-6380. Fax: (415) 476-5233. email: vale{at}phy.ucsf.edu

Cell migration occurs through the protrusion of the actin-enrichedlamella. Here, we investigated the effects of RNAi depletionof $~$ 90 proteins implicated in actin function on lamella formationin Drosophila S2 cells. Similar to in vitro reconstitution studiesof actin-based Listeria movement, we find that lamellae formationrequires a relatively small set of proteins that participatein actin nucleation (Arp2/3 and SCAR), barbed end capping (cappingprotein), filament depolymerization (cofilin and Aip1), andactin monomer binding (profilin and cyclase-associated protein).Lamellae are initiated by parallel and partially redundant signalingpathways involving Rac GTPases and the adaptor protein Nck,which stimulate SCAR, an Arp2/3 activator. We also show thatRNAi of three proteins (kette, Abi, and Sra-1) known to copurifywith and inhibit SCAR in vitro leads to SCAR degradation, revealinga novel function of this protein complex in SCAR stability.Our results have identified an essential set of proteins involvedin actin dynamics during lamella formation in Drosophila S2cells.

Key Words: actin; lamella; polymerization; SCAR; cytokinesis

The online version of this article includes supplemental material.

Abbreviations used in this paper: CAP, cyclase-associated protein;con A, concanavalin A; VASP, vasodilator-stimulated phosphoprotein.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

Related Article

We can deplete it, we have the technology: Nicole LeBrasseur
J. Cell Biol. 2003 162: 958. [Full Text] [PDF]

This article has been cited by other articles:

Shim, J., Lee, S.-M., Lee, M. S., Yoon, J., Kweon, H.-S., Kim, Y.-J. (2010). Rab35 Mediates Transport of Cdc42 and Rac1 to the Plasma Membrane during Phagocytosis. Mol. Cell. Biol. 30: 1421-1433 [Abstract] [Full Text]
Fujiwara, I., Remmert, K., Hammer, J. A. III (2010). Direct Observation of the Uncapping of Capping Protein-capped Actin Filaments by CARMIL Homology Domain 3. J. Biol. Chem. 285: 2707-2720 [Abstract] [Full Text]
Dharmalingam, E., Haeckel, A., Pinyol, R., Schwintzer, L., Koch, D., Kessels, M. M., Qualmann, B. (2009). F-BAR Proteins of the Syndapin Family Shape the Plasma Membrane and Are Crucial for Neuromorphogenesis. J. Neurosci. 29: 13315-13327 [Abstract] [Full Text]
Lin, T.-Y., Huang, C.-H., Kao, H.-H., Liou, G.-G., Yeh, S.-R., Cheng, C.-M., Chen, M.-H., Pan, R.-L., Juang, J.-L. (2009). Abi plays an opposing role to Abl in Drosophila axonogenesis and synaptogenesis. Development 136: 3099-3107 [Abstract] [Full Text]
Vale, R. D., Spudich, J. A., Griffis, E. R. (2009). Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells. JCB 186: 727-738 [Abstract] [Full Text]
Ku, H.-Y., Wu, C.-L., Rabinow, L., Chen, G.-C., Meng, T.-C. (2009). Organization of F-Actin via Concerted Regulation of Kette by PTP61F and dAbl. Mol. Cell. Biol. 29: 3623-3632 [Abstract] [Full Text]
Brawley, C. M., Rock, R. S. (2009). Unconventional myosin traffic in cells reveals a selective actin cytoskeleton. Proc. Natl. Acad. Sci. USA 106: 9685-9690 [Abstract] [Full Text]
Dyachok, J., Shao, M.-R., Vaughn, K., Bowling, A., Facette, M., Djakovic, S., Clark, L., Smith, L. (2008). Plasma Membrane-Associated SCAR Complex Subunits Promote Cortical F-Actin Accumulation and Normal Growth Characteristics in Arabidopsis Roots. Mol Plant 1: 990-1006 [Abstract] [Full Text]
LeClaire, L. L. III, Baumgartner, M., Iwasa, J. H., Mullins, R. D., Barber, D. L. (2008). Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments. JCB 182: 647-654 [Abstract] [Full Text]
Kueh, H. Y., Charras, G. T., Mitchison, T. J., Brieher, W. M. (2008). Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. JCB 182: 341-353 [Abstract] [Full Text]
Trammell, M. A., Mahoney, N. M., Agard, D. A., Vale, R. D. (2008). Mob4 plays a role in spindle focusing in Drosophila S2 cells. J. Cell Sci. 121: 1284-1292 [Abstract] [Full Text]
Le Clainche, C., Carlier, M.-F. (2008). Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration. Physiol. Rev. 88: 489-513 [Abstract] [Full Text]
Korobova, F., Svitkina, T. (2008). Arp2/3 Complex Is Important for Filopodia Formation, Growth Cone Motility, and Neuritogenesis in Neuronal Cells. Mol. Biol. Cell 19: 1561-1574 [Abstract] [Full Text]
Abou-Kheir, W., Isaac, B., Yamaguchi, H., Cox, D. (2008). Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2. J. Cell Sci. 121: 379-390 [Abstract] [Full Text]
Perroud, P.-F., Quatrano, R. S. (2008). BRICK1 Is Required for Apical Cell Growth in Filaments of the Moss Physcomitrella patens but Not for Gametophore Morphology. Plant Cell 20: 411-422 [Abstract] [Full Text]
Koo, I. C., Ohol, Y. M., Wu, P., Morisaki, J. H., Cox, J. S., Brown, E. J. (2008). Role for lysosomal enzyme -hexosaminidase in the control of mycobacteria infection. Proc. Natl. Acad. Sci. USA 105: 710-715 [Abstract] [Full Text]
Jani, K., Schock, F. (2007). Zasp is required for the assembly of functional integrin adhesion sites. JCB 179: 1583-1597 [Abstract] [Full Text]
Kaizuka, Y., Douglass, A. D., Varma, R., Dustin, M. L., Vale, R. D. (2007). Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells. Proc. Natl. Acad. Sci. USA 104: 20296-20301 [Abstract] [Full Text]
Richardson, B. E., Beckett, K., Nowak, S. J., Baylies, M. K. (2007). SCAR/WAVE and Arp2/3 are crucial for cytoskeletal remodeling at the site of myoblast fusion. Development 134: 4357-4367 [Abstract] [Full Text]
Sidani, M., Wessels, D., Mouneimne, G., Ghosh, M., Goswami, S., Sarmiento, C., Wang, W., Kuhl, S., El-Sibai, M., Backer, J. M., Eddy, R., Soll, D., Condeelis, J. (2007). Cofilin determines the migration behavior and turning frequency of metastatic cancer cells. JCB 179: 777-791 [Abstract] [Full Text]
Huang, C.-H., Lin, T.-Y., Pan, R.-L., Juang, J.-L. (2007). The Involvement of Abl and PTP61F in the Regulation of Abi Protein Localization and Stability and Lamella Formation in Drosophila S2 Cells. J. Biol. Chem. 282: 32442-32452 [Abstract] [Full Text]
Vidali, L., Augustine, R. C., Kleinman, K. P., Bezanilla, M. (2007). Profilin Is Essential for Tip Growth in the Moss Physcomitrella patens. Plant Cell 19: 3705-3722 [Abstract] [Full Text]
Kile, B. T., Panopoulos, A. D., Stirzaker, R. A., Hacking, D. F., Tahtamouni, L. H., Willson, T. A., Mielke, L. A., Henley, K. J., Zhang, J.-G., Wicks, I. P., Stevenson, W. S., Nurden, P., Watowich, S. S., Justice, M. J. (2007). Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia. Blood 110: 2371-2380 [Abstract] [Full Text]
Carlier, M.-F., Pantaloni, D. (2007). Control of Actin Assembly Dynamics in Cell Motility. J. Biol. Chem. 282: 23005-23009 [Full Text]
Deeks, M. J., Rodrigues, C., Dimmock, S., Ketelaar, T., Maciver, S. K., Malho, R., Hussey, P. J. (2007). Arabidopsis CAP1 a key regulator of actin organisation and development. J. Cell Sci. 120: 2609-2618 [Abstract] [Full Text]
Ren, N., Charlton, J., Adler, P. N. (2007). The flare Gene, Which Encodes the AIP1 Protein of Drosophila, Functions to Regulate F-Actin Disassembly in Pupal Epidermal Cells. Genetics 176: 2223-2234 [Abstract] [Full Text]
Chaudhry, F., Guerin, C., von Witsch, M., Blanchoin, L., Staiger, C. J. (2007). Identification of Arabidopsis Cyclase-associated Protein 1 as the First Nucleotide Exchange Factor for Plant Actin. Mol. Biol. Cell 18: 3002-3014 [Abstract] [Full Text]
Griffis, E. R., Stuurman, N., Vale, R. D. (2007). Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. JCB 177: 1005-1015 [Abstract] [Full Text]
Clark, M. G., Amberg, D. C. (2007). Biochemical and Genetic Analyses Provide Insight Into the Structural and Mechanistic Properties of Actin Filament Disassembly by the Aip1p Cofilin Complex in Saccharomyces cerevisiae. Genetics 176: 1527-1539 [Abstract] [Full Text]
Bakal, C., Aach, J., Church, G., Perrimon, N. (2007). Quantitative Morphological Signatures Define Local Signaling Networks Regulating Cell Morphology. Science 316: 1753-1756 [Abstract] [Full Text]
Gates, J., Mahaffey, J. P., Rogers, S. L., Emerson, M., Rogers, E. M., Sottile, S. L., Van Vactor, D., Gertler, F. B., Peifer, M. (2007). Enabled plays key roles in embryonic epithelial morphogenesis in Drosophila. Development 134: 2027-2039 [Abstract] [Full Text]
Cai, L., Makhov, A. M., Bear, J. E. (2007). F-actin binding is essential for coronin 1B function in vivo. J. Cell Sci. 120: 1779-1790 [Abstract] [Full Text]
Caracino, D., Jones, C., Compton, M., Saxe, C. L. III (2007). The N-Terminus of Dictyostelium Scar Interacts with Abi and HSPC300 and Is Essential for Proper Regulation and Function. Mol. Biol. Cell 18: 1609-1620 [Abstract] [Full Text]
Goshima, G., Wollman, R., Goodwin, S. S., Zhang, N., Scholey, J. M., Vale, R. D., Stuurman, N. (2007). Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells. Science 316: 417-421 [Abstract] [Full Text]
Mongiu, A. K., Weitzke, E. L., Chaga, O. Y., Borisy, G. G. (2007). Kinetic-structural analysis of neuronal growth cone veil motility. J. Cell Sci. 120: 1113-1125 [Abstract] [Full Text]
Legg, J. A., Bompard, G., Dawson, J., Morris, H. L., Andrew, N., Cooper, L., Johnston, S. A., Tramountanis, G., Machesky, L. M. (2007). N-WASP Involvement in Dorsal Ruffle Formation in Mouse Embryonic Fibroblasts. Mol. Biol. Cell 18: 678-687 [Abstract] [Full Text]
Yamazaki, D., Oikawa, T., Takenawa, T. (2007). Rac-WAVE-mediated actin reorganization is required for organization and maintenance of cell-cell adhesion. J. Cell Sci. 120: 86-100 [Abstract] [Full Text]
Miyoshi, T., Tsuji, T., Higashida, C., Hertzog, M., Fujita, A., Narumiya, S., Scita, G., Watanabe, N. (2006). Actin turnover-dependent fast dissociation of capping protein in the dendritic nucleation actin network: evidence of frequent filament severing. JCB 175: 947-955 [Abstract] [Full Text]
Cram, E. J., Shang, H., Schwarzbauer, J. E. (2006). A systematic RNA interference screen reveals a cell migration gene network in C. elegans. J. Cell Sci. 119: 4811-4818 [Abstract] [Full Text]
Brieher, W. M., Kueh, H. Y., Ballif, B. A., Mitchison, T. J. (2006). Rapid actin monomer-insensitive depolymerization of Listeria actin comet tails by cofilin, coronin, and Aip1. JCB 175: 315-324 [Abstract] [Full Text]
Wu, J.-Q., Sirotkin, V., Kovar, D. R., Lord, M., Beltzner, C. C., Kuhn, J. R., Pollard, T. D. (2006). Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. JCB 174: 391-402 [Abstract] [Full Text]
Okada, K., Ravi, H., Smith, E. M., Goode, B. L. (2006). Aip1 and Cofilin Promote Rapid Turnover of Yeast Actin Patches and Cables: A Coordinated Mechanism for Severing and Capping Filaments. Mol. Biol. Cell 17: 2855-2868 [Abstract] [Full Text]
Steffen, A., Faix, J., Resch, G. P., Linkner, J., Wehland, J., Small, J. V., Rottner, K., Stradal, T. E.B. (2006). Filopodia Formation in the Absence of Functional WAVE- and Arp2/3-Complexes. Mol. Biol. Cell 17: 2581-2591 [Abstract] [Full Text]
Suetsugu, S., Kurisu, S., Oikawa, T., Yamazaki, D., Oda, A., Takenawa, T. (2006). Optimization of WAVE2 complex-induced actin polymerization by membrane-bound IRSp53, PIP3, and Rac. JCB 173: 571-585 [Abstract] [Full Text]
Williams, M. J., Wiklund, M.-L., Wikman, S., Hultmark, D. (2006). Rac1 signalling in the Drosophila larval cellular immune response. J. Cell Sci. 119: 2015-2024 [Abstract] [Full Text]
Mohri, K., Ono, K., Yu, R., Yamashiro, S., Ono, S. (2006). Enhancement of Actin-depolymerizing Factor/Cofilin-dependent Actin Disassembly by Actin-interacting Protein 1 Is Required for Organized Actin Filament Assembly in the Caenorhabditis elegans Body Wall Muscle. Mol. Biol. Cell 17: 2190-2199 [Abstract] [Full Text]
Semba, S., Iwaya, K., Matsubayashi, J., Serizawa, H., Kataba, H., Hirano, T., Kato, H., Matsuoka, T., Mukai, K. (2006). Coexpression of Actin-Related Protein 2 and Wiskott-Aldrich Syndrome Family Verproline-Homologous Protein 2 in Adenocarcinoma of the Lung. Clin. Cancer Res. 12: 2449-2454 [Abstract] [Full Text]
Clark, M. G., Teply, J., Haarer, B. K., Viggiano, S. C., Sept, D., Amberg, D. C. (2006). A Genetic Dissection of Aip1p's Interactions Leads to a Model for Aip1p-Cofilin Cooperative Activities. Mol. Biol. Cell 17: 1971-1984 [Abstract] [Full Text]
Djakovic, S., Dyachok, J., Burke, M., Frank, M. J., Smith, L. G. (2006). BRICK1/HSPC300 functions with SCAR and the ARP2/3 complex to regulate epidermal cell shape in Arabidopsis. Development 133: 1091-1100 [Abstract] [Full Text]
Minin, A. A., Kulik, A. V., Gyoeva, F. K., Li, Y., Goshima, G., Gelfand, V. I. (2006). Regulation of mitochondria distribution by RhoA and formins. J. Cell Sci. 119: 659-670 [Abstract] [Full Text]
Abou Kheir, W., Gevrey, J.-C., Yamaguchi, H., Isaac, B., Cox, D. (2005). A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J. Cell Sci. 118: 5369-5379 [Abstract] [Full Text]
Di Nardo, A., Cicchetti, G., Falet, H., Hartwig, J. H., Stossel, T. P., Kwiatkowski, D. J. (2005). Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function. Proc. Natl. Acad. Sci. USA 102: 16263-16268 [Abstract] [Full Text]
Lord, M., Laves, E., Pollard, T. D. (2005). Cytokinesis Depends on the Motor Domains of Myosin-II in Fission Yeast but Not in Budding Yeast. Mol. Biol. Cell 16: 5346-5355 [Abstract] [Full Text]
Nishita, M., Tomizawa, C., Yamamoto, M., Horita, Y., Ohashi, K., Mizuno, K. (2005). Spatial and temporal regulation of cofilin activity by LIM kinase and Slingshot is critical for directional cell migration. JCB 171: 349-359 [Abstract] [Full Text]
Weisswange, I., Bretschneider, T., Anderson, K. I. (2005). The leading edge is a lipid diffusion barrier. J. Cell Sci. 118: 4375-4380 [Abstract] [Full Text]
Dean, S. O., Rogers, S. L., Stuurman, N., Vale, R. D., Spudich, J. A. (2005). Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Proc. Natl. Acad. Sci. USA 102: 13473-13478 [Abstract] [Full Text]
Jia, Z., Barbier, L., Stuart, H., Amraei, M., Pelech, S., Dennis, J. W., Metalnikov, P., O'Donnell, P., Nabi, I. R. (2005). Tumor Cell Pseudopodial Protrusions: LOCALIZED SIGNALING DOMAINS COORDINATING CYTOSKELETON REMODELING, CELL ADHESION, GLYCOLYSIS, RNA TRANSLOCATION, AND PROTEIN TRANSLATION. J. Biol. Chem. 280: 30564-30573 [Abstract] [Full Text]
Williams, M. J., Ando, I., Hultmark, D. (2005). Drosophila melanogaster Rac2 is necessary for a proper cellular immune response. GENES CELLS 10: 813-823 [Abstract] [Full Text]
Zhang, X., Dyachok, J., Krishnakumar, S., Smith, L. G., Oppenheimer, D. G. (2005). IRREGULAR TRICHOME BRANCH1 in Arabidopsis Encodes a Plant Homolog of the Actin-Related Protein2/3 Complex Activator Scar/WAVE That Regulates Actin and Microtubule Organization. Plant Cell 17: 2314-2326 [Abstract] [Full Text]
Maddox, A. S., Habermann, B., Desai, A., Oegema, K. (2005). Distinct roles for two C. elegans anillins in the gonad and early embryo. Development 132: 2837-2848 [Abstract] [Full Text]
Field, C. M., Coughlin, M., Doberstein, S., Marty, T., Sullivan, W. (2005). Characterization of anillin mutants reveals essential roles in septin localization and plasma membrane integrity. Development 132: 2849-2860 [Abstract] [Full Text]
Kovar, D. R., Wu, J.-Q., Pollard, T. D. (2005). Profilin-mediated Competition between Capping Protein and Formin Cdc12p during Cytokinesis in Fission Yeast. Mol. Biol. Cell 16: 2313-2324 [Abstract] [Full Text]
Riol-Blanco, L., Sanchez-Sanchez, N., Torres, A., Tejedor, A., Narumiya, S., Corbi, A. L., Sanchez-Mateos, P., Rodriguez-Fernandez, J. L. (2005). The Chemokine Receptor CCR7 Activates in Dendritic Cells Two Signaling Modules That Independently Regulate Chemotaxis and Migratory Speed. J. Immunol. 174: 4070-4080 [Abstract] [Full Text]
Stramer, B., Wood, W., Galko, M. J., Redd, M. J., Jacinto, A., Parkhurst, S. M., Martin, P. (2005). Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration. JCB 168: 567-573 [Abstract] [Full Text]
Basu, D., Le, J., El-Essal, S. E.-D., Huang, S., Zhang, C., Mallery, E. L., Koliantz, G., Staiger, C. J., Szymanski, D. B. (2005). DISTORTED3/SCAR2 Is a Putative Arabidopsis WAVE Complex Subunit That Activates the Arp2/3 Complex and Is Required for Epidermal Morphogenesis. Plant Cell 17: 502-524 [Abstract] [Full Text]
Grove, M., Demyanenko, G., Echarri, A., Zipfel, P. A., Quiroz, M. E., Rodriguiz, R. M., Playford, M., Martensen, S. A., Robinson, M. R., Wetsel, W. C., Maness, P. F., Pendergast, A. M. (2004). Abi2-Deficient Mice Exhibit Defective Cell Migration, Aberrant Dendritic Spine Morphogenesis, and Deficits in Learning and Memory. Mol. Cell. Biol. 24: 10905-10922 [Abstract] [Full Text]
Ling, S.-C., Fahrner, P. S., Greenough, W. T., Gelfand, V. I. (2004). Transport of Drosophila fragile X mental retardation protein-containing ribonucleoprotein granules by kinesin-1 and cytoplasmic dynein. Proc. Natl. Acad. Sci. USA 101: 17428-17433 [Abstract] [Full Text]
Naim, V., Imarisio, S., Di Cunto, F., Gatti, M., Bonaccorsi, S. (2004). Drosophila Citron Kinase Is Required for the Final Steps of Cytokinesis. Mol. Biol. Cell 15: 5053-5063 [Abstract] [Full Text]
Li, Y., Sorefan, K., Hemmann, G., Bevan, M. W. (2004). Arabidopsis NAP and PIR Regulate Actin-Based Cell Morphogenesis and Multiple Developmental Processes. Plant Physiol. 136: 3616-3627 [Abstract] [Full Text]
Shandala, T., Gregory, S. L., Dalton, H. E., Smallhorn, M., Saint, R. (2004). Citron Kinase is an essential effector of the Pbl-activated Rho signalling pathway in Drosophila melanogaster. Development 131: 5053-5063 [Abstract] [Full Text]
Bompard, G., Caron, E. (2004). Regulation of WASP/WAVE proteins: making a long story short. JCB 166: 957-962 [Abstract] [Full Text]
Schroter, R. H., Lier, S., Holz, A., Bogdan, S., Klambt, C., Beck, L., Renkawitz-Pohl, R. (2004). kette and blown fuse interact genetically during the second fusion step of myogenesis in Drosophila. Development 131: 4501-4509 [Abstract] [Full Text]
Basu, D., El-Assal, S. E.-D., Le, J., Mallery, E. L., Szymanski, D. B. (2004). Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex subunit SRA1 during leaf epidermal development. Development 131: 4345-4355 [Abstract] [Full Text]
Brembu, T., Winge, P., Seem, M., Bones, A. M. (2004). NAPP and PIRP Encode Subunits of a Putative Wave Regulatory Protein Complex Involved in Plant Cell Morphogenesis. Plant Cell 16: 2335-2349 [Abstract] [Full Text]
Bogdan, S., Grewe, O., Strunk, M., Mertens, A., Klambt, C. (2004). Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila. Development 131: 3981-3989 [Abstract] [Full Text]
Mohri, K., Vorobiev, S., Fedorov, A. A., Almo, S. C., Ono, S. (2004). Identification of Functional Residues on Caenorhabditis elegans Actin-interacting Protein 1 (UNC-78) for Disassembly of Actin Depolymerizing Factor/Cofilin-bound Actin Filaments. J. Biol. Chem. 279: 31697-31707 [Abstract] [Full Text]
Echarri, A., Lai, M. J., Robinson, M. R., Pendergast, A. M. (2004). Abl Interactor 1 (Abi-1) Wave-Binding and SNARE Domains Regulate Its Nucleocytoplasmic Shuttling, Lamellipodium Localization, and Wave-1 Levels. Mol. Cell. Biol. 24: 4979-4993 [Abstract] [Full Text]
Bertling, E., Hotulainen, P., Mattila, P. K., Matilainen, T., Salminen, M., Lappalainen, P. (2004). Cyclase-associated Protein 1 (CAP1) Promotes Cofilin-induced Actin Dynamics in Mammalian Nonmuscle Cells. Mol. Biol. Cell 15: 2324-2334 [Abstract] [Full Text]
Marcy, Y., Prost, J., Carlier, M.-F., Sykes, C. (2004). Forces generated during actin-based propulsion: A direct measurement by micromanipulation. Proc. Natl. Acad. Sci. USA 101: 5992-5997 [Abstract] [Full Text]
Wear, M. A., Cooper, J. A. (2004). Capping Protein Binding to S100B: IMPLICATIONS FOR THE "TENTACLE" MODEL FOR CAPPING THE ACTIN FILAMENT BARBED END. J. Biol. Chem. 279: 14382-14390 [Abstract] [Full Text]
Gautreau, A., Ho, H.-y. H., Li, J., Steen, H., Gygi, S. P., Kirschner, M. W. (2004). Purification and architecture of the ubiquitous Wave complex. Proc. Natl. Acad. Sci. USA 101: 4379-4383 [Abstract] [Full Text]