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Published 11 April 2005. doi:10.1083/jcb.200502104
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 169, Number 1, 35-47
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Article

Proteomic and genomic characterization of chromatin complexes at a boundary



Alan J. Tackett1, David J. Dilworth2,3, Megan J. Davey1, Michael O'Donnell1,4, John D. Aitchison2,3, Michael P. Rout1, and Brian T. Chait1

1 The Rockefeller University, New York, NY 10021
2 Institute for Systems Biology, Seattle, WA 98103
3 University of Alberta, Edmoton, Alberta T6G2H7, Canada
4 Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021

Correspondence to Brian T. Chait: chait{at}rockefeller.edu

We have dissected specialized assemblies on the Saccharomyces cerevisiae genome that help define and preserve the boundaries that separate silent and active chromatin. These assemblies contain characteristic stretches of DNA that flank particular regions of silent chromatin, as well as five distinctively modified histones and a set of protein complexes. The complexes consist of at least 15 chromatin-associated proteins, including DNA pol {varepsilon}, the Isw2-Itc1 and Top2 chromatin remodeling proteins, the Sas3-Spt16 chromatin modifying complex, and Yta7, a bromodomain-containing AAA ATPase. We show that these complexes are important for the faithful maintenance of an established boundary, as disruption of the complexes results in specific, anomalous alterations of the silent and active epigenetic states.

M.J. Davey's present address is The University of Western Ontario, London, Ontario N6A 5B8, Canada.

Abbreviations used in this paper: ChIP, chromatin immunoprecipitation; MS, mass spectrometry; PrA, protein A.


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