Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 11201K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hanson, P. I. Articles by Heuser, J. E. Search for Related Content PubMed PubMed Citation Articles by Hanson, P. I. Articles by Heuser, J. E. Related Collections Related In this Issue article Social Bookmarking                            What's this?

Plasma membrane deformation by circular arrays of ESCRT-III protein filaments

Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110

Correspondence to P. Hanson: phanson22{at}wustl.edu

Endosomal sorting complex required for transport III (ESCRT-III) proteins function in multivesicular body biogenesis and viral budding. They are recruited from the cytoplasm to the membrane, where they assemble into large complexes. We used "deep-etch" electron microscopy to examine polymers formed by the ESCRT-III proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B). When overexpressed, these proteins target to endosomes and the plasma membrane. Both hSnf7 proteins assemble into regular approximately 5-nm filaments that curve and self-associate to create circular arrays. Binding to a coexpressed adenosine triphosphate hydrolysis–deficient mutant of VPS4B draws these filaments together into tight circular scaffolds that bend the membrane away from the cytoplasm to form buds and tubules protruding from the cell surface. Similar buds develop in the absence of mutant VPS4B when hSnf7-1 is expressed without its regulatory C-terminal domain. We demonstrate that hSnf7 proteins form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding. We suggest that ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Related In this Issue article

ESCRT rings around the membrane

Nicole LeBrasseur
J. Cell Biol. 2008 180: 248. [Full Text] [PDF]

This article has been cited by other articles:

• Nickerson, D. P., West, M., Henry, R., Odorizzi, G. (2010). Regulators of Vps4 ATPase Activity at Endosomes Differentially Influence the Size and Rate of Formation of Intralumenal Vesicles. Mol. Biol. Cell 21: 1023-1032 [Abstract] [Full Text]  
• Shestakova, A., Hanono, A., Drosner, S., Curtiss, M., Davies, B. A., Katzmann, D. J., Babst, M. (2010). Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol. Biol. Cell 21: 1059-1071 [Abstract] [Full Text]  
• Pawliczek, T., Crump, C. M. (2009). Herpes Simplex Virus Type 1 Production Requires a Functional ESCRT-III Complex but Is Independent of TSG101 and ALIX Expression. J. Virol. 83: 11254-11264 [Abstract] [Full Text]  
• Tran, J. H., Chen, C.-J., Emr, S., Schekman, R. (2009). Cargo sorting into multivesicular bodies in vitro. Proc. Natl. Acad. Sci. USA 106: 17395-17400 [Abstract] [Full Text]  
• Xiao, J., Chen, X.-W., Davies, B. A., Saltiel, A. R., Katzmann, D. J., Xu, Z. (2009). Structural Basis of Ist1 Function and Ist1-Did2 Interaction in the Multivesicular Body Pathway and Cytokinesis. Mol. Biol. Cell 20: 3514-3524 [Abstract] [Full Text]  
• Vaccari, T., Rusten, T. E., Menut, L., Nezis, I. P., Brech, A., Stenmark, H., Bilder, D. (2009). Comparative analysis of ESCRT-I, ESCRT-II and ESCRT-III function in Drosophila by efficient isolation of ESCRT mutants. J. Cell Sci. 122: 2413-2423 [Abstract] [Full Text]  
• Popov, S., Popova, E., Inoue, M., Gottlinger, H. G. (2009). Divergent Bro1 Domains Share the Capacity To Bind Human Immunodeficiency Virus Type 1 Nucleocapsid and To Enhance Virus-Like Particle Production. J. Virol. 83: 7185-7193 [Abstract] [Full Text]  
• Wollert, T., Yang, D., Ren, X., Lee, H. H., Im, Y. J., Hurley, J. H. (2009). The ESCRT machinery at a glance. J. Cell Sci. 122: 2163-2166 [Full Text]  
• McDonald, B., Martin-Serrano, J. (2009). No strings attached: the ESCRT machinery in viral budding and cytokinesis. J. Cell Sci. 122: 2167-2177 [Abstract] [Full Text]  
• Aoh, Q. L., Castle, A. M., Hubbard, C. H., Katsumata, O., Castle, J. D. (2009). SCAMP3 Negatively Regulates Epidermal Growth Factor Receptor Degradation and Promotes Receptor Recycling. Mol. Biol. Cell 20: 1816-1832 [Abstract] [Full Text]  
• Agromayor, M., Carlton, J. G., Phelan, J. P., Matthews, D. R., Carlin, L. M., Ameer-Beg, S., Bowers, K., Martin-Serrano, J. (2009). Essential Role of hIST1 in Cytokinesis. Mol. Biol. Cell 20: 1374-1387 [Abstract] [Full Text]  
• Bajorek, M., Morita, E., Skalicky, J. J., Morham, S. G., Babst, M., Sundquist, W. I. (2009). Biochemical Analyses of Human IST1 and Its Function in Cytokinesis. Mol. Biol. Cell 20: 1360-1373 [Abstract] [Full Text]  
• Rodriguez-Galan, O., Galindo, A., Hervas-Aguilar, A., Arst, H. N. Jr., Penalva, M. A. (2009). Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III. J. Biol. Chem. 284: 4404-4412 [Abstract] [Full Text]  
• Lindas, A.-C., Karlsson, E. A., Lindgren, M. T., Ettema, T. J. G., Bernander, R. (2008). A unique cell division machinery in the Archaea. Proc. Natl. Acad. Sci. USA 105: 18942-18946 [Abstract] [Full Text]  
• Falguieres, T., Luyet, P.-P., Bissig, C., Scott, C. C., Velluz, M.-C., Gruenberg, J. (2008). In Vitro Budding of Intralumenal Vesicles into Late Endosomes Is Regulated by Alix and Tsg101. Mol. Biol. Cell 19: 4942-4955 [Abstract] [Full Text]  
• Lee, H. H., Elia, N., Ghirlando, R., Lippincott-Schwartz, J., Hurley, J. H. (2008). Midbody Targeting of the ESCRT Machinery by a Noncanonical Coiled Coil in CEP55. Science 322: 576-580 [Abstract] [Full Text]  
• Lata, S., Schoehn, G., Jain, A., Pires, R., Piehler, J., Gottlinger, H. G., Weissenhorn, W. (2008). Helical Structures of ESCRT-III Are Disassembled by VPS4. Science 321: 1354-1357 [Abstract] [Full Text]  
• McCullough, J., Fisher, R. D., Whitby, F. G., Sundquist, W. I., Hill, C. P. (2008). ALIX-CHMP4 interactions in the human ESCRT pathway. Proc. Natl. Acad. Sci. USA 105: 7687-7691 [Abstract] [Full Text]  
• Shim, S., Merrill, S. A., Hanson, P. I. (2008). Novel Interactions of ESCRT-III with LIP5 and VPS4 and their Implications for ESCRT-III Disassembly. Mol. Biol. Cell 19: 2661-2672 [Abstract] [Full Text]  
• LeBrasseur, N. (2008). ESCRT rings around the membrane. JCB 180: 248-248 [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents