We demonstrate an additional step and a positive feedback loop in leukocyte accumulation on inflamed endothelium. Leukocytes in shear flow bind to adherent leukocytes through L-selectin/ligand interactions and subsequently bind downstream and roll on inflamed endothelium, purified E-selectin, P-selectin, L-selectin, VCAM-1, or peripheral node addressin. Thus adherent leukocytes nucleate formation of strings of rolling cells and synergistically enhance leukocyte accumulation. Neutrophils, monocytes, and activated T cell lines, but not peripheral blood T lymphocytes, tether to each other through L-selectin. L-selectin is not involved in direct binding to either E- or P-selectin and is not a major counterreceptor of endothelial selectins. Leukocyte-leukocyte tethers are more tolerant to high shear than direct tethers to endothelial selectins and, like other L-selectin-mediated interactions, require a shear threshold. Synergism between leukocyte-leukocyte and leukocyte-endothelial interactions introduces novel regulatory mechanisms in recruitment of leukocytes in inflammation.
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November 01 1996
Interactions through L-selectin between leukocytes and adherent leukocytes nucleate rolling adhesions on selectins and VCAM-1 in shear flow.
R Alon,
R Alon
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
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R C Fuhlbrigge,
R C Fuhlbrigge
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
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E B Finger,
E B Finger
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
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T A Springer
T A Springer
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
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R Alon
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
R C Fuhlbrigge
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
E B Finger
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
T A Springer
Center for Blood Research, Department of Pathology, Boston, Massachusetts 02115, USA.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1996) 135 (3): 849–865.
Citation
R Alon, R C Fuhlbrigge, E B Finger, T A Springer; Interactions through L-selectin between leukocytes and adherent leukocytes nucleate rolling adhesions on selectins and VCAM-1 in shear flow.. J Cell Biol 1 November 1996; 135 (3): 849–865. doi: https://doi.org/10.1083/jcb.135.3.849
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