Damage recognition by repair/checkpoint factors is the critical first step of the DNA damage response. DNA double strand breaks (DSBs) activate checkpoint signaling and are repaired by nonhomologous end-joining (NHEJ) and homologous recombination (HR) pathways. However, in vivo kinetics of the individual factor responses and the mechanism of pathway choice are not well understood. We report cell cycle and time course analyses of checkpoint activation by ataxia-telangiectasia mutated and damage site recruitment of the repair factors in response to laser-induced DSBs. We found that MRN acts as a DNA damage marker, continuously localizing at unrepaired damage sites. Damage recognition by NHEJ factors precedes that of HR factors. HR factor recruitment is not influenced by NHEJ factor assembly and occurs throughout interphase. Damage site retention of NHEJ factors is transient, whereas HR factors persist at unrepaired lesions, revealing unique roles of the two pathways in mammalian cells.
DNA double strand breaks (DSBs) that are induced by endogenous and exogenous causes have deleterious effects on genome stability. To ensure efficient repair, DSBs activate checkpoint signaling to halt the cell cycle and inhibit DNA replication (Shiloh, 2003). Ataxia-telangiectasia (A-T) mutated (A-TM), a member of the PI3 kinase-related protein kinases (PIKKs; Abraham, 2004), is a major DSB signal transducer and is critical for activating the G1/S, S, and G2/M checkpoints. A-TM phosphorylates several target proteins that are critical for checkpoint signaling, such as Chk2 (Ahn et al., 2000). DSBs are repaired by two major pathways: nonhomologous end joining (NHEJ) and homologous recombination (HR), which require distinct sets of factors (Lieber et al., 2003; West, 2003). DNA-dependent protein kinase catalytic subunit (DNA–PKcs) and Ku are recruited to DNA ends to initiate the NHEJ cascade, which is followed by recruitment of the XRCC4–ligase IV complex. Rad51 and replication protein A (RPA) are essential factors for the HR pathway in vertebrate cells and are recruited to single-stranded DNA (ssDNA) regions at broken DNA ends to catalyze invasion of ssDNA into the homologous DNA template (Sung, 1994; Baumann et al., 1996). The trimeric complex Mre11–Rad50–Nbs1 (MRN) functions at an early stage of HR (Paull and Gellert, 1998) as well as in A-TM–mediated checkpoint activation in mammalian cells (Carson et al., 2003; Uziel et al., 2003; Costanzo et al., 2004; Horejsi et al., 2004; Lee and Paull, 2004, 2005). These checkpoint and repair factors all contribute to protecting the genome against DSBs. However, the in vivo kinetics and cell cycle specificity of damage response by these factors and the relationship between the two DSB repair pathways are not well understood.
Previously, we used 532 nm of second harmonic pulsed Q-switched Nd:YAG (yttrium-aluminum garnet) laser microbeam irradiation (“laser scissors”) to induce multiple DNA breaks at defined regions in the cell nucleus. We demonstrated the immediate recruitment of several DSB factors, including MRN, and the sister chromatid cohesion factor cohesin to the site of damage (Kim et al., 2002). Cohesin recruitment was recently confirmed by chromatin cross-linking and immunoprecipitation analysis of endonuclease cut sites in yeast (Ström et al., 2004; Ünal et al., 2004). By using the laser system, we report a cell cycle and time course analysis of checkpoint signaling and DSB factor assembly at the damage sites. Our results demonstrate different timing and durations of damage recognition by MRN, NHEJ, and HR factors, providing new insight into the unique roles of MRN and the two repair pathways in mammalian cells.
Results And Discussion
Laser-induced damage causes checkpoint activation and cell cycle delay in human cells
Chk2 was phosphorylated at the site of damage, which is consistent with the immediate recruitment of A-TM and A-TM and Rad3 related (A-TR) to the laser-induced damage site (Fig. 1 A). Phosphorylation of Chk2 occurred in A-TM–inactive A-T cells (Fig. 1 B). This is a result of the redundant function of other PIKKs such as A-TR (Shiloh, 2003), which was suppressed by further treatment with wortmannin (Fig. 1 B). Chk2 was phosphorylated immediately at the damage sites in both G1 and S/G2 phases (Fig. 1 C). Chk2 phosphorylation was observed as foci throughout the nucleus by 2 h postdamage induction, which was particularly prominent in G1 phase. Thus, Chk2 phosphorylation initially occurrs at the damage sites and subsequently spreads to the nucleus. Similar spreading was observed with UV laser–induced DSB damage (Lukas et al., 2003). The results demonstrate that Nd:YAG laser–induced damage evokes a checkpoint response and that checkpoint signaling initially occurs at the damage sites and is followed by dissemination throughout the nucleus.
Consistent with the presence of DSBs, H2AX phosphorylation (γH2AX) was observed at the damage site immediately after damage induction (Fig. 2 A). γH2AX persisted even after 24 h after damage (ad), indicating the presence of unrepaired damage in the irradiated region. Concomitantly, a significant cell cycle delay was observed (Fig. 2 B). Although the adjacent undamaged cells continued to divide, damaged cells remained in interphase for >40 h, and some eventually entered mitosis (Fig. 2 B, Caffeine [−]). When cells were treated with caffeine, however, all the damaged cells underwent and successfully completed mitosis within the first 18 h in a manner similar to the adjacent undamaged cells (Fig. 2 B, Caffeine [+]). These results demonstrate that although some DSBs are sustained, laser-induced damage is not lethal and causes checkpoint-dependent cell cycle delay.
Immediate and independent recruitment of MRN and NHEJ factors in G1 and S/G2 phases
We observed that Mre11 and Ku were recruited immediately to the damage sites (Fig. 3 ; Kim et al., 2002). Neither Ku nor Mre11 was depleted by laser-induced DSBs because comparable amounts of Ku and Mre11 were detected at two successively induced damage sites (Fig. 3 A).
Mre11 was recruited to damage sites in both G1 and S/G2 phases (Fig. 3 B), as were its partners Rad50 and Nbs1 (not depicted). Similarly, the NHEJ factors Ku, DNA–PKcs, and ligase IV immediately clustered to damage sites in both G1 and S/G2 phases, supporting the notion that NHEJ is active in both phases (Fig. 3 B and Fig. S1 A, available at http//:www.jcb.org/cgi/content/full/jcb.200411083/DC1; Rothkamm et al., 2003). Despite similar timing, recruitment of MRN and Ku were independent of each other (Fig. 3 C). Mutation of Mre11 or Nbs1 did not affect the localization of Ku. Conversely, Mre11 recruitment was intact in Ku knockout cells.
Although Ku binding to the endonuclease-induced damage site was demonstrated in vivo by chromatin immunoprecipitation analysis (Martin et al., 1999), the localization of NHEJ factors at ionizing radiation (IR)–induced damage sites has not been cytologically detected (Jakob et al., 2002; Lisby et al., 2004). This was thought to be caused by subdetectable amounts of recruited NHEJ factors (Jakob et al., 2002; Lisby et al., 2004). The number of DNA breaks that are induced by the Nd:YAG laser should be substantial and are likely comparable with those induced by a UV laser (in combination with halogenated nucleotides). This is based on γH2AX staining and DNA end labeling by terminal deoxynucleotidyltransferase in both systems (Fig. 2 A; Paull et al., 2000; Kim et al., 2002; Lukas et al., 2003; Bradshaw et al., 2005). A conservative estimate of UV laser energy was reported to be >80 Gy (Paull et al., 2000), which was estimated to cause ∼2,800 breaks per nucleus (Bradshaw et al., 2005). Thus, clustering of these breaks to one small region in the nucleus appears to allow detection of the weak immunofluorescent signals of NHEJ factors at the individual DNA ends.
NHEJ factor assembly at the damage sites is transient
Interestingly, Ku was no longer found at the damage sites at 8 h ad, whereas Mre11 persisted (Fig. 3 D). The retention time of Ku was found to be between 4 and 6 h (not depicted). Thus, Ku leaves damage sites before the completion of DNA repair. A similar observation was made with DNA–PKcs (not depicted). For comparison, recruitment of the DNA break sensor poly(ADP-ribose) polymerase 1 (PARP-1) was examined. Binding of PARP-1 to DNA breaks triggers auto-(ADP-ribosyl)ation, resulting in its dissociation from the lesion (Lindahl et al., 1995). PARP-1 accumulated at the damage site immediately after damage but became undetectable by 2 h ad, revealing the factor-specific kinetics of damage site recognition.
Delayed but persistent HR factor recruitment is not affected by NHEJ factor assembly and occurs throughout interphase
In S/G2 phase, the HR factor Rad51 was recruited to damage sites, although its recruitment was distinctly delayed (Fig. 4) . Within 20 min ad, the accumulation of Mre11 was observed as a continuous line corresponding to the area of laser ablation, with no evidence of Rad51 accumulation. By 2 h, however, Rad51 was clearly visible as discontinuous speckles along the damaged area that colocalized with Mre11. Rad51 recruitment was first observed between 30 min and 1 h ad (Fig. 5 A). A similar delay of Rad52 focus formation was observed in response to γ irradiation in yeast (Lisby et al., 2004). The IR-induced foci (IRIF) of MRN and Rad51 were shown to be nonoverlapping and mutually exclusive (Maser et al., 1997). Because laser-irradiated regions contain multiple DNA breaks, MRN and Rad51 possibly bind to different DNA ends in different stages of processing, which is similar to the overlapping signals of MRN and Rad52 that are observed at IR-induced damage sites in Saccharomyces cerevisiae (Lisby et al., 2004). The morphological changes of the damage site are likely caused by partial repair and damage clustering that is similar to what was observed with localized α particle irradiation (Aten et al., 2004). A few bright fluorescent signals of Mre11 and Rad51 remained at the damage sites at 24 h ad, indicating that unrepaired DSB lesions are persistently marked by MRN and Rad51 in addition to γH2AX (Figs. 2 A and 4). This is in marked contrast to the transient retention of Ku (Fig. 3 D).
It was thought that competition occurs between the NHEJ and HR pathways, in which DNA end binding of Ku precludes the assembly of HR factors at damage sites (Van Dyck et al., 1999; Fukushima et al., 2001; Pierce et al., 2001; Allen et al., 2002; Karanjawala et al., 2002). However, the delay of Rad51 recruitment is not caused by competition with NHEJ factor assembly because the timing of Rad51 recruitment does not change in either Ku- or DNA–PKcs-deficient cells (Fig. 5 A). Because RPA recruitment is also delayed (Fig. S1 B and not depicted), DNA end processing to produce sufficient ssDNA may be the rate-limiting step for HR factor assembly in vivo. The timing of MRN, Ku, and Rad51 recruitment is unaffected by wortmannin (Fig. S1 C).
Because HR prefers the use of sister chromatids as templates in vertebrate cells, HR repair is considered to be the main pathway for postreplicative repair during late S/G2 phase (Takata et al., 1998; Sonoda et al., 1999; Rothkamm et al., 2003). Surprisingly, however, Rad51 accumulated at the laser-induced damage sites in G1 phase with kinetics similar to those in S/G2 phase (Fig. 5 B, Fig. S1 D, and Fig. S2 B, available at http://www.jcb.org/cgi/content/full/jcb.200411083/DC1) even though the expression level of Rad51 protein was low in G1 phase (Fig. 5 C). A similar observation was made with RPA (Fig. S1 B). The results indicate that Rad51 and RPA are capable of detecting the processed ssDNA ends at DSB sites throughout interphase in human cells. Interestingly, a similar G1 phase recruitment of RPA, but not Rad51, was observed in yeast (Lisby et al., 2004). The presence of dense, laser-induced DSBs may explain the observed Rad51 signal in G1 phase, which was not previously detected as IRIF (Tashiro et al., 2000; Lisby et al., 2004). Curiously, several studies using lasers suggested that IRIF formation involves more than initial damage site factor recruitment. For example, although Nbs1 is required for IRIF formation by Mre11–Rad50 (Carney et al., 1998), it is not required for recruitment of Mre11–Rad50 to laser-induced damage sites (Kim et al., 2002). H2AX is required for Nbs1 focus formation (Celeste et al., 2002) but is dispensable for the recruitment of Nbs1 to UV/BrdU-induced damage sites (Celeste et al., 2003). Thus, although S/G2-specific Rad51 IRIF may reflect ongoing HR, Rad51 may nonetheless associate with ssDNA at damage sites without initiating the actual strand invasion process until the homologous templates are available.
Our findings demonstrate the distinct kinetics of DNA damage recognition by different DSB repair factors in vivo. Our results reveal the unique behavior of MRN, which is different from both NHEJ and HR factors, as a DNA damage marker constitutively associated with unrepaired lesions (Fig. 5 D). We found that Ku localization at damage sites is transient, which is in contrast to the prolonged retention of Rad51. The distinct timing of recruitment between NHEJ and HR factors clearly demonstrates that NHEJ factor assembly precedes that of HR factors even in S/G2 phases. This is consistent with previous reports suggesting that NHEJ repair precedes HR repair (Delacote et al., 2002; Frank-Vaillant and Marcand, 2002). Thus, the results suggest that NHEJ and HR are not two competing parallel pathways. Rather, NHEJ serves as an immediate early repair pathway, whereas HR factors make a more prolonged attempt to repair persistent DNA lesions. These partially overlapping but complementary roles of the two pathways could explain the compensatory (originally interpreted as “competition”) and cooperative functions of NHEJ and HR in the maintenance of genome integrity (Takata et al., 1998; Fukushima et al., 2001; Pierce et al., 2001; Delacote et al., 2002; Couëdel et al., 2004; Mills et al., 2004).
Although confined to a small area (∼0.01% of the nuclear volume), one must be cautious because cellular responses to such extensive Nd:YAG laser–induced damage may differ from those after more physiologicaly relevant low dose IR, as the observed protein clustering may be specific to high density DSBs. However, there is a strong similarity of laser-evoked responses (both checkpoint activation and repair factor recruitment) to the known and/or predicted responses to DSB damage by conventional methods without lethality. This, along with the fact that different factors exhibit distinct timing and duration of damage site clustering, strongly argue that this type of laser system is useful for defining a temporal framework of DNA damage recognition and a nuclear-wide response of DSB factors in mammalian cells.
Materials And Methods
Cell culture and synchronization
Wild-type human fibroblast IMR-90 cells and isogenic human glioma cell lines lacking (M059J) or expressing (M059K) DNA–PKcs were obtained from the American Type Culture Collection. Mre11 mutant fibroblast A-T-like disorder (A-TLD) 2 cells were derived from an A-TLD patient (Stewart et al., 1999). The human A-T fibroblast cell line (GM02052D) was obtained from Coriell Cell Repositories. HeLa and other human cells were cultured as previously described (Kim et al., 2002) or according to the suppliers' instructions. Ku80 knockout and wild-type mouse embryonic fibroblasts (MEFs) were cultured as described previously (Bailey et al., 1999). Cells were grown on etched grid coverslips (Bellco Biotechnology) that were attached to the bottom of preholed tissue culture plates.
HeLa cells were synchronized to S and G2 phases by a double thymidine block (Gregson et al., 2001). For G1 phase, cells in mitosis after a single thymidine block were continuously monitored to confirm their division and were subjected to laser microirradiation 5 h after M phase identification (Fig. S2 B).
Cells were preincubated with 200 μM wortmannin (AG Scientific, Inc.) 1 h before DNA damage induction. Wortmannin treatment was maintained until the cells were fixed. For caffeine treatment, cells that were released from double thymidine block were preincubated with or without 2 mM caffeine (Sigma-Aldrich) for 1 h before damage induction (Blasina et al., 1999).
Laser damage was induced as previously described (Kim et al., 2002). In brief, 532 nm of the second harmonic of a pulsed Nd:YAG laser beam (∼2–3 μJ/pulse energy after objective; ∼4–6 ns pulse duration; 7.5 Hz; just above the estimated threshold values of optical breakdown in water [Venugopalan et al., 2002]) was focused through a 100× Ph3 Neofluar oil-immersion objective (NA1.3; Carl Zeiss MicroImaging, Inc.) in a confocal system (model LSM410; Carl Zeiss MicroImaging, Inc.). Unlike a UV laser, the Nd:YAG laser can be focused to a 200–300 nm spot, which is calculated to affect a minimum region of ∼24 kb of a 30-nm chromatin fiber with a packing ratio of 40. Scattering should be minimum because 532 nm (visible green) is not absorbed by the cell. An EM study confirmed that the damage is structurally confined to the focused area (Berns et al., 1998). Damage is most likely caused by ionization (optical breakdown) of the medium (water) and plasma formation rather than heat because of the extremely short duration of the pulse (5 ns; Berns et al., 1998; Venugopalan et al., 2002). The expansion of cavitation bubbles that was caused by plasma generated transient photomechanical pressure, which resulted in breakage of molecular bonding and created numerous DNA breaks in the confined area. At least three independent experiments for each antibody staining were performed. Five to seven cells were damaged in one plate, and three to four plates were subjected to laser damage in each experiment. For the data presented, 100% of cells exhibited the same staining patterns with a given antibody.
After laser microirradiation, cells were fixed in 4% PFA (10 min at 4°C) and stained with antibodies. The staining procedure was described previously (Kim et al., 2002). Imaging was performed on a microscope (model IX81; Olympus) coupled with a CCD camera (model FV II; Olympus) using a 100× Ph3 UPlanFI oil objective (NA 1.3; Olympus).
Rabbit pAb against the human Rad51 protein was raised against the bacterially expressed protein. Commercial antibodies that were used include mouse mAbs specific for Mre11, Rad50, and A-TM (GeneTex), Ku70 (Novus Biologicals), PARP-1 (Trevigen), DNA–PKcs (Abcam Limited), rabbit pAbs specific for hMre11 (Oncogene Research Products), ligase IV and RPA (Chemicon International), phospho-Chk2 (Thr68; Cell Signaling Technology), and pAb and mAb anti-Nbs1 (Novus Biologicals and BD Biosciences, respectively). The specificity of antibodies against A-TM was confirmed (Fig. S2 A). Goat anti-A-TR pAb was from Santa Cruz Biotechnology, Inc. Donkey anti–mouse IgG and anti–goat IgG conjugated with AlexaFluor488 and goat anti–rabbit IgG conjugated with AlexaFluor546 were from Molecular Probes. Cy3-conjugated goat anti–rabbit IgG was from Jackson ImmunoResearch Laboratories.
Online supplemental material
Supplemental figures provide additional immunostaining data. Fig. S1 shows colocalization of Ku and Mre11 at the damage site; RPA recruitment in G1 and S/G2 phases; the lack of effect of wortmannin treatment on Ku, Mre11, and Rad51 recruitment; and colocalization of Mre11 and Rad51 at the damage site in G1 phase cells. Fig. S2 shows the specificity of the anti-A-TM antibody by the absence of A-T cell staining and shows the distinction of cell cycle stages by proliferating cell nuclear antigen staining. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200411083/DC1.
We would like to thank Drs. Shunichi Takeda, Stephen West, Stephen Jackson, and Michael Lieber for helpful comments on the manuscript.
This work was supported in part by the Bioarchitect Research Program (RIKEN) and grants-in-aid from the Japan Society for the Promotion of Science and the Ministry of Education, Culture, Sports, Science, and Technology of Japan to H. Kurumizaka, and by grants from the National Institutes of Health (CA50519 to D.J. Chen and CA100710 to K. Yokomori) and the Department of Defense Breast Cancer Research Program (DAMD17-03-1-0436 to K. Yokomori). K. Yokomori was a Leukemia and Lymphoma Society Scholar.
Jong-Soo Kim's present address is Neuroscience Research Institute, Gachon Medical School, Namdong-gu, Incheon 405-760, Korea.
Abbreviations used in this paper: ad, after damage; A-T, ataxia-telangiectasia; A-TLD, A-T-like disorder; A-TM, A-T mutated; A-TR, A-T and Rad3 related; DNA–PKcs, DNA-dependent protein kinase catalytic subunit; DSB, DNA double strand break; HR, homologous recombination; IR, ionizing radiation; IRIF, IR-induced foci; Nd:YAG, neodymium/yttrium-aluminum garnet; NHEJ, nonhomologous end joining; MRN, Mre11–Rad50–Nbs1; PARP-1, poly(ADP-ribose) polymerase 1; PIKK, PI3 kinase-related protein kinase; RPA, replication protein A; ssDNA, single-stranded DNA.
- Submitted: 15 November 2004
- Accepted: 21 June 2005