July 2014 | Volume 206, No. 2
News
In This Issue
In Focus
Lipids help epithelia stand tallPalmitoylation and phosphoinositides target ankyrin-G/βII-spectrin network to lateral membranes.
People & Ideas
Gohta Goshima: Questing for answers on the mitotic spindleGoshima studies spindle assembly and microtubule dynamics.
Review
Review
Research Articles
Reports
The SUN protein UNC-84 is required only in force-bearing cells to maintain nuclear envelope architectureSUN-KASH bridges that connect the nucleoskeleton to the cytoskeleton are only required to maintain nuclear envelope spacing in cells subjected to increased mechanical forces, such as muscle cells.
The general amino acid control pathway regulates mTOR and autophagy during serum/glutamine starvationTo meet their metabolic needs, starved cells first activate autophagy, but activation in parallel of the general amino acid control pathway increases amino acid uptake, leading to reactivation of mTOR and down-regulation of autophagy.
Articles
DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesisDeubiquitination of Rad18 drives its localization to sites of DNA damage and formation of the Rad18–SHPRH complexes necessary for error-free lesion bypass.
Klar ensures thermal robustness of oskar localization by restraining RNP motilityWhen temperature fluctuation threatens the fidelity of Drosophila oogenesis, Klar restrains posterior-ward translocation of oskar mRNA, thereby adapting the rate of oskar delivery to the capacity of the anchoring machinery.
AGO3 Slicer activity regulates mitochondria–nuage localization of Armitage and piRNA amplificationThe endonuclease AGO3 and mitochondria-associated protein Zucchini together control the dynamic subcellular localization of Armitage between mitochondria and germline granules to regulate secondary piRNA amplification.
Direct kinetochore–spindle pole connections are not required for chromosome segregationIn the absence of continuous K-fiber attachment between each kinetochore and the spindle pole, one or more additional mechanisms dependent on dynein-mediated kinetochore transport exist to ensure proper chromosome segregation during mitosis.
Force on spindle microtubule minus ends moves chromosomesAfter the loss of continuous spindle microtubule attachment to the spindle pole, a previously undescribed mechanism of chromosome transport, powered by dynein pulling on minus ends of severed microtubules, repairs spindle architecture and integrity.
Excess centrosomes disrupt endothelial cell migration via centrosome scatteringCentrosome–microtubule interactions during interphase are important for centrosome clustering and cell polarity.
Ankyrin-G palmitoylation and βII-spectrin binding to phosphoinositide lipids drive lateral membrane assemblyPalmitoylation of ankyrin-G and interaction of βII-spectrin with phosphoinositides are necessary for ankyrin-G–βII-spectrin localization in membrane subdomains during lateral membrane assembly in columnar epithelial cells.
Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activitySirtuin-mediated deacetylation of the catalytic subunit of mitochondrial complex V increases complex activity.
A RAB5/RAB4 recycling circuitry induces a proteolytic invasive program and promotes tumor disseminationRAB5A and RAB4 promote breast tumor cell dissemination by controlling the trafficking of proteins necessary for localized invadosome formation.
