April 2015 | Volume 209, No. 1
In This Issue
People & Ideas
- A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front–back polarity
A group of phosphorylatable serine residues within the nonhelical domain of NMII-B controls the ability of NMII-B to generate stable migratory front–rear polarity.
- Excision of translesion synthesis errors orchestrates responses to helix-distorting DNA lesions
An Msh2/Msh6-dependent DNA repair mechanism mitigates the mutagenicity of photolesions and induces cell cycle responses by excising incorrect nucleotides incorporated by postreplicative translesion synthesis.
- Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
Long-term imaging via microfluidic chambers shows that two minus end–directed motors, dynein and Klp2, work in parallel at distinct subcellular structures to promote efficient nuclear congression.
- A role for disulfide bonding in keratin intermediate filament organization and dynamics in skin keratinocytes
Disulfide bonds involving cysteine 367 in K14 play a crucial role in the assembly, dynamics, and organization of K14-containing filaments in epidermal keratinocytes.
- Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway
The function of Sac2/INPP5F in the endocytic pathway and its activity as a 4-phosphatase suggest that Sac2/INPP5F and OCRL may cooperate in the sequential dephosphorylation of PI(4,5)P2 in a partnership that mimics that of the two phosphatase modules of synaptojanin.
- Spatiotemporal control of phosphatidylinositol 4-phosphate by Sac2 regulates endocytic recycling
Sac2 (INPP5F) is a phosphoinositide 4-phosphatase that specifically hydrolyzes PI(4)P and regulates endocytic recycling.
- TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone
TMEM231, a functional component of the MKS complex at the ciliary transition zone, is mutated in orofaciodigital syndrome type 3 and Meckel syndrome.
- TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse
TAGLN2 stabilizes cortical F-actin and thereby maintains F-actin contents at the immunological synapse, which allows T cell activation following T cell receptor stimulation.
- Open source software for quantification of cell migration, protrusions, and fluorescence intensities
ADAPT is an ImageJ plug-in that can be used for rapid whole-cell analysis of time-lapse videos, thereby providing data on cell morphology, membrane velocity, and temporal changes in any fluorescent protein of interest at the cell periphery, as exemplified by the morphological characterization of cellular blebs.