April 2016 | Volume 213, No. 1
People & Ideas
- Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton
Fungal and human septins can distinguish between different degrees of micron-scale curvature in cells, suggesting that this property of the septin cytoskeleton provides a cell with a mechanism to sense its local shape.
- Dynamic formation of ER–PM junctions presents a lipid phosphatase to regulate phosphoinositides
Dickson et al. find that the ER membrane lipid phosphatase Sac1 localizes to ER–plasma membrane (PM) contact sites and acts as a cellular sensor and controller of PM phosphoinositide homeostasis.
- Doa1 targets ubiquitinated substrates for mitochondria-associated degradation
Wu et al. show that Doa1 recognizes and recruits ubiquitinated mitochondrial outer-membrane proteins to the Cdc48–proteasome degradation pathway. Doa1 deficiency sensitizes cells to mitochondrial oxidative stress.
- Sigma1 receptors inhibit store-operated Ca2+ entry by attenuating coupling of STIM1 to Orai1
Sigma1 receptors inhibit store-operated Ca2+ entry and reduce the Ca2+ content of the intracellular stores. These effects are regulated by Sigma1 receptor ligands. A ligand-dependent interaction between Sigma1R and STIM1 reduces STIM1 association with Orai1 channels.
- P53- and mevalonate pathway–driven malignancies require Arf6 for metastasis and drug resistance
The mevalonate pathway (MVP) is a metabolic pathway associated with tumor invasiveness and is known to prenylate proteins, but which prenylated proteins are critical for MVP-driven cancers is unknown. Hashimoto et al. show that MVP-driven cancers require activation of the GTPase Arf6 for invasion and that the MVP substrate Rab11 is required for Arf6 activation.
- Kindlin-2 directly binds actin and regulates integrin outside-in signaling
Bledzka et al. show that kindlin-2 binds actin via its F0 domain, and mutation of this site diminishes cell spreading, revealing a new mechanism by which kindlin-2 regulates cellular responses.
- Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence
The authors find that matrix metalloproteinase MT1-MMP is enriched at the plasma membrane of macrophage podosomes, where it persists beyond podosome lifetime and, through binding to the subcortical actin cytoskeleton, forms subcellular signposts that facilitate podosome reformation.
- A versatile multivariate image analysis pipeline reveals features of Xenopus extract spindles
The authors describe automated image and data analysis tools that reveal architectural principles of the Xenopus egg extract spindle, allow for rapid, unbiased assessment of spindle phenotypes, and can be adapted to analyze other subcellular structures such as nuclei.