May 2016 | Volume 213, No. 3
People & Ideas
- Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network
Crevenna et al. examine the mechanism by which secretory cargoes are segregated at the trans-Golgi network (TGN) for release into the extracellular space. The authors demonstrate that Ca2+-dependent changes in Cab45 oligomerization mediate sorting of specific cargo molecules at the TGN.
- A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends
The Astrin/SKAP complex regulates mitotic chromosome alignment and centrosome integrity, but previous work found conflicting results for SKAP function. Here, Kern et al. demonstrate that a previously unappreciated short SKAP isoform mediates mitotic spindle positioning at astral microtubule plus ends.
- Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype
Rao et al. show that during migration, neurons contain a small population of centrosome-unattached microtubules in the leading process that are capable of sliding. Increasing the proportion of centrosome-unattached microtubules alters neuronal morphology, migration path, and microtubule behavior in the leading process.
- TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum
Santos et al. show that TANGO1 and a TANGO1-like protein, TALI, bind each other and function together as receptors to export bulky ApoB-containing lipid particles from the endoplasmic reticulum. However, TANGO1-mediated export of bulky collagens by the same cells is TALI independent.
- Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures
Epithelial polarity establishment involves transcytosis of podocalyxin to the apical domain, but its route and regulation are unclear. Here, Mrozowska and Fukuda demonstrate that different Rabs and Rab effectors coordinate podocalyxin transport during polarization in 2D and 3D structures.
- Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity
The cytoskeletal adapter protein talin plays a prominent role in adhesive structures connecting integrins to the actin cytoskeleton. In this work, Kumar et al. use a novel talin sensor to measure talin tension and provide insights into focal adhesion force transmission and mechanosensitivity.
- RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border
Rho/ROCK signaling is essential to maintain the integrity of the endothelial barrier, but the contributions of specific Rho GTPase family members are unclear. Here, Marcos-Ramiro et al. show that RhoB specifically regulates intracellular trafficking of the Rho GTPase Rac1 and thereby controls endothelial barrier restoration during inflammation.