Files in this Data Supplement:
- Supplemental Materials (PDF)
- Video 1
Composite movie showing parentalCapture spans 4 h (approximately one frame every 10 min) from the moment of serum withdrawal (promoting ciliary growth) from confluent cultures. The number of stable cilia relative to the total number of cilia seen in these fields of view is indicated. Bar, 20 µm. RPE1WTand KDM3Anull(line 2, herein named KDM3Acr22.2) cells transiently expressing 5HT6-EGFP as ciliary marker.
- Video 2
Close-ups from Video 1 showing stableSome still frames are shown in Fig. 6 A. RPE1WTand unstable KDM3Anullcilia.
- Video 3
Composite movie showing primary wild-type andCaptures show cilia of confluent cultures maintained in the absence of serum. Kdm3aΔJC/ΔJCMEFs transfected with ARL13B-mKATE2.
- Video 4
Composite movie shows parental RPE1Capture spans 115 min (approximately one frame every 3 min), after which most of RPE1 WTand KDM3Anullcells transiently expressing 5HT6-EGFP during ciliary resorption (from time frame 5). WTcilia are resorbed but that of KDM3Anullremain.
- Video 5
RPE1The right panel shows the KDM3Ai2-EGFP single channel. WTcells cotransfected with KDM3Ai2-EGFP and ARL13B-mKATE2 (merged channels) show incorporation of tagged KDM3A to the cilium.