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Article

Cyclin B3 promotes anaphase I onset in oocyte meiosis

Mehmet E. Karasu, Nora Bouftas, View ORCID ProfileScott Keeney  Correspondence email, View ORCID ProfileKatja Wassmann  Correspondence email
Mehmet E. Karasu
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NYLouis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan-Kettering Cancer Center, New York, NYHoward Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, NY
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Nora Bouftas
Institut de Biologie Paris Seine, Sorbonne Université, Paris, FranceDevelopmental Biology Lab, Sorbonne Université, Centre National de la Recherche Scientifique UMR7622, Paris, France
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Scott Keeney
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NYLouis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan-Kettering Cancer Center, New York, NYHoward Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, NY
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  • For correspondence: s-keeney@ski.mskcc.org
Katja Wassmann
Institut de Biologie Paris Seine, Sorbonne Université, Paris, FranceDevelopmental Biology Lab, Sorbonne Université, Centre National de la Recherche Scientifique UMR7622, Paris, France
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  • ORCID record for Katja Wassmann
  • For correspondence: katja.wassmann@upmc.fr
DOI: 10.1083/jcb.201808091 | Published February 5, 2019
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Data supplements

  • Files in this Data Supplement:

    • Supplemental Materials (PDF)
    • Video 1 -
      Progression through meiosis I in control oocyte. A Ccnb3+/− oocyte incubated with SiR-DNA to visualize chromosomes. Acquisitions were started at GVBD + 6:30 (hours:minutes). 11 z-sections with 3-µm steps were taken to follow chromosome movements, and shown are overlays of the stack. Acquisitions of the far-red and DIC channel were done every 20 min; 21 time points are shown. Related to Fig. 2 A. This is a representative video from 42 oocytes from six independent experiments.
    • Video 2 -
      Progression through meiosis I in Ccnb3−/− oocyte. A Ccnb3−/− oocyte incubated with SiR-DNA to visualize chromosomes. Acquisitions were started at GVBD + 6:30 (hours:minutes). 11 z-sections with 3-µm steps were taken to follow chromosome movements, and shown are overlays of the stack. Acquisitions of the far-red and DIC channel were done every 20 min, 21 time points are shown. Related to Fig. 2 A. This is a representative video from 63 oocytes from six independent experiments.
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Cyclin B3 promotes anaphase I onset in oocyte meiosis
Mehmet E. Karasu, Nora Bouftas, Scott Keeney, Katja Wassmann
J Cell Biol Feb 2019, jcb.201808091; DOI: 10.1083/jcb.201808091

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The Journal of Cell Biology: 218 (2)

Current Issue

February 4, 2019
Volume 218, No. 2

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  • Genetics
  • Development
  • Cell Cycle and Division

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