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Figure S1. Effect of other cup mutant combinations on the localization of oskar mRNA and Btz protein. Localization of oskar mRNA is normal in cup1/cup1355 and cup1/cup4 egg chambers during stages 1-7. (A) Stage 5 yw egg chamber. (B) Stage 6 cup1/cup1355 egg chamber. (C) Stage 7 cup1/cup4 egg chamber. During stage 8, oskar mRNA localization to the posterior pole is disrupted. (D) Late stage 8 yw egg chamber. (E) Stage 8 cup1/cup1355 egg chamber. (F) Early stage 8 cup1/cup4 egg chamber. (G) Late stage 8 cup1/cup44 egg chamber. Btz protein is not localized to the oocyte in cup1/cup4506 females during stage 5 (H), stage 7 (I), and stage 9 (J). Btz protein is not localized to the oocyte in cup1/cup4 females during stage 6 (K), stage 8 (L), and stage 9 (M). Actin is red. Bar, 10 &microm.