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Video 1 (3.4 MB)
Real-time imaging of an en face sequential exocytic event that occurred toward microvessels inside an intact islet. The islet was immersed in a solution containing 0.7 mM SRB, and images were acquired every 0.8 s beginning 150 s after the onset of stimulation with 20 mM glucose. Fusion of the secondary granule occurred 36 s after the primary event, and both granules eventually flattened out within the plasma membrane.