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Figure S1. mif2-2 mutants undergo spindle extension in the absence of chromatid disjunction. WT (JBY428), mif2-2 (JBY450), and mif2-2mad2-�� (JBY548) TRP1-GFP strains were released from G1 at 35°C. Time points were processed using {alpha}-tubulin immunofluorescence to determine kinetics of budding, spindle extension, and TRP1-GFP separation.