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Figure S1. Lithium facilitates clearance of mutant huntingtin aggregates, an effect not seen with sodium chloride. (A and B) Stable inducible PC12 cells expressing EGFP-HDQ74 were induced with doxycycline for 8 h, then transgene expression was switched off for 120 h (by removing doxycycline) (A) or 170 h (B), with (+) or without (-) 10 mM LiCl or 10 mM NaCl. Confocal microscopy images show EGFP-positive cells with aggregates (green, indicated by white arrows). Bar, 20 ┬Ám. (C) The percentage of EGFP-positive cells with aggregates as in B were expressed as odds ratio, compared to the control untreated cells (170 h off).