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Figure S2. Lithium induces autophagy to facilitate clearance of mutant proteins. (A and B) COS-7 (A) or HeLa (B) cells were transfected with pEGFP-LC3 for 4 h and treated with or without 1 or 10 mM LiCl for 2 h, and analyzed by fluorescence microscopy. Cells were pretreated with or without 1 or 10 mM LiCl for 24 h before transfection. The effects of treatment on the percentage of EGFP-positive cells with EGFP-LC3 vesicles were expressed as odds ratios. (C) Fluorescent microscope images of EGFP-HDQ74-positive COS-7 cells with aggregates, treated with (+) or without (-) 10 mM 3-MA, 10 mM LiCl or both for 48 h as in Fig. 2 G. Images were taken in high intensity (top) and low intensity (middle) to denote the number EGFP-positive transfected cells and aggregates (indicated by arrows), respectively. Nuclei are stained with DAPI (bottom). Bar, 20 ┬Ám.