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Video 2
Fluorescence time-lapse video microscopy of spindle MTs after anaphase was induced in Xorbit-depleted Xenopus laevis egg extract. Spindle MTs rapidly depolymerize, and the two spindle poles collapse in this sequence. One of the poles splits apart, generating two asters. MTs were fluorescently labeled with X-rhodamine. Frames were collected every 30 s and are displayed at 10 frames/s. Total time of this video is 12 min, and fixed samples are shown in Fig. 3 B.