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Video 3
Time-lapse movie of FP-Q69YH5 (green) transiently expressed in a mitotic HeLa cell stained with Hoechst 33342 to mark chromatin (red). Z series images (5 x 1 µM sections) were taken every 5 min as the cell progressed from prophase through telophase and are presented here as 2D projections of the 3D datasets. The display rate is 12 frames/s, and still images are shown in Fig. 4 G. Bar, 30 µM.