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Figure S3. 14-3-3 proteins bind phosphorylated S939 and S981 residues. Several isoforms of GST-14-3-3 proteins were used to affinity purify proteins from HEK293 cells in the presence of phosphorylated or nonphosphorylated S939 (A) and S981 (B) tuberin peptides. Affinity-purified complexes were analyzed by Western blotting to detect the amount of tuberin interacting with 14-3-3.