Table S2. PCR conditions

Target Cycle conditions
Fragment S; fragment A 1 min at 95�C, 20 s at 96�C, 15 s at 97�C, 20 × (22 s at 96�C, 1 min at 67.5�C– 0.4�C/cycle, 3 min 15 s at 72�C), 16 × (22 s at 96�C, 1 min at 60�C, 3 min 15 s at 72�C), 10 min at 72�C
LC8 promoter; LC8 terminator 1 min at 95�C, 20 s at 96�C, 15 s at 97�C, 20 × (22 s at 96�C, 50 s at 68�C–0.5�C/cycle, 1 min 20 s at 72�C), 15 × (20 s at 96�C, 50 s at 62�C, 1 min 20 s at 72�C), 10 min at 72�C
3xHA tag 8 min at 96�C, 25 × (45 s at 96�C, 30 s at 63�C, 20 s at 72�C), 10 min at 72�C
RT-PCR 7 min at 96�C, 35 × (35 s at 95�C, 35 s at 59.5�C, 50 s at 72�C), 10 min at 72�C