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Figure S4. Tm treatment causes cytokinesis delay. Experiments were performed according to the schematic in Fig. S3 A, and cells were collected for immunoblot analysis, probing for Pgk1p (loading control) and Clb2p (A), Cdc14p-GFP visualization (B), Tub1p-GFP visualization (C), and AlexaFluor546-phalloidin staining to visualize actin patch localization (D). Blue indicates DAPI staining. Bars, 2 ┬Ám.