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Figure S3. BMP6 induces tube formation by endothelial cells in a 3D collagen gel assay. MECs suspended in DME were diluted in a solution of rat type 1 collagen and plated in an uncoated 24-well plate. BMP6 was added to the MEC/collagen mixture before collagen polymerization. Tube formation was observed at 24 h. (A) High resolution light microscopic image of vacuoles (arrow) in MECs treated with BMP6 in 3D culture in vitro. (B) Cross-section image of tubes formed by MECs treated with BMP6 in 3D culture (arrowheads point to the tube lumen).