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Figure S2. The Bid sequence is a target for ubiquitination. HA ubiquitin was coexpressed in HeLa cells with the TAP empty vector, tBid-N TAP, and tBid-N TAP lacking the first 23 amino acids of Bid (Δ23). Proteins were purified on the TAP tag, separated by SDS-PAGE, and probed by immunoblotting for ubiquitin and Bid with anti-HA and -Bid antibodies, respectively. Data show that ubiquitination is specific for Bid because the TAP tag alone does not carry ubiquitin and the deletion mutation of Bid abrogates ubiquitination.