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Figure S3. Effects of point mutations on tBid-N stability. Alanine substitution mutations were generated in HA tBid-N TEV 30. Wild-type and mutant proteins were expressed in HeLa cells, which were incubated for the indicated time periods with CHX with or without MG132. Total cell lysates (TCLs) were examined by anti-HA immunoblotting for levels of HA tBid-N TEV 30. Lines indicate samples tested together in one experiment.