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Figure S5. Time course of tBid-N disappearance. MCF-7 Bid RNAi cells expressing Myc Bid GFP were stimulated with TNF-α for 2, 4, or 8 h or not (control), stained to detect mitochondria (MitoTracker) and the N-terminal Myc tag, and examined by CLSM. The 4-h time point represents cells in which the GFP signal localized at mitochondria (∼40% of the population). At 8 h, the frequency of cells with such localization was ∼80%. In the other cells, localization was as depicted for the 2-h time point.