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Video 1
MTOC translocation is prevented in dynamitin-disrupted cells. MTOC movement toward the IS is not observed upon T cell–APC contact in dynamitin-disrupted cells (green). Video sequences are displayed at 4 frames per second. SEE-pulsed Raji APC (CMCTR-preloaded, red/orange) were maintained in HBSS (2% BSA) at 37°C (5% CO2). T cells were then added, and images were captured every 30 s using a TCS SP5 confocal laser scanning unit attached to a DMI6000 (HCX PL APO 63×/1.40-0.6 NA oil λBL objective). Video 2 is shown in Fig. 1 F (middle).