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Video 2
MTOC translocation is achieved at early times upon T-APC contact. The MTOC moves toward the contact area with the APC early upon first contact in Jurkat T cells overexpressing paxillin-GFP (green). Video sequences are displayed at 4 frames per second. SEE-pulsed Raji APC (CMCTR-preloaded, red/orange) were maintained in HBSS (2% BSA) at 37°C (5% CO2). T cells were then added, and images were captured every 30 s using a TCS SP confocal laser scanning unit attached to a DMIRBE (HCX PL APO 63×/1.32-0.60 NA oil objective). Video 1 is shown in Fig 1 F (top).