[HELP with Viewing Videos]

Video 6
Cilengitide and osteopontin induce the formation of highly dynamic actin-rich protrusions at the cell front. Cells were transfected with GFP-actin and allowed to grow to confluence. Confluent monolayers were wounded with a plastic pipette tip and the cells were allowed to migrate into the wound in the presence of 0.5 µg/ml osteopontin. The cells were imaged by time-lapse confocal microscopy. Images were captured at five frames per second over a period of 5 min and the video was generated from these. The video plays at 100 frames per second.