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Video 10
GFP-RCP is localized to the tip of the extending pseudopod as cells migrate on a 3D matrix. Cells were transfected with GFP-RCP and plated onto cell-derived matrix in the presence of 1 ┬ÁM cilengitide and imaged by confocal microscopy. Images were captured at one frame per second over a period of 100 s and a video was generated. This video represents a region corresponding to the white box in Fig. 5 C. The Video plays at 100 frames per second.