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Figure S3. Time course of association of α5β1 with RCP after addition of cilengitide. (A) Cells were transfected with GFP-RCP, and then incubated in the absence (BAS) or presence (CIL) of 1 µM cilengitide for the indicated times. Lysates were immunoprecipitated using monoclonal antibodies recognizing GFP and immobilized material was analyzed for the presence of α5 integrin and GFP-RCP by Western blotting. (B) Paxillin is not associated with RCP immunoprecipitates. Cells were transfected with GFP or GFP-RCP, and then incubated in the absence (BAS) or presence (CIL) of 1 µM cilengitide for 20 min. Lysates were immunoprecipitated using monoclonal antibodies recognizing GFP and immobilized material was analyzed for the presence of α5 integrin, paxillin, and GFP-RCP by Western blotting.