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Figure S1. Efficacy of siRNAs. (A–C) Cells were transfected with siRNAs targeting Memo (A and B), mDia1 (B), or RhoA (C) and cDNA constructs (Myc-tagged Memo or Flag-tagged mDia1 [ΔN3]) 48 h before cell lysis and Western blotting analysis using the indicated antibodies. Shc, GSK-3β, and Akt serve as loading controls. (D and E)Memo controls the localization of specific lamellipodial markers. Immunolocalization of α-actinin-1 (D), Arp3, and cortactin (E) in SKBR3 cells expressing control or Memo siRNA. Filamentous actin (F-actin) was revealed with Alexa Fluor 546 phalloidin. In Memo-depleted cells, α-actinin-1 lamellipodial labeling is reduced, and cortactin is restricted to the plasma membrane, whereas Arp3 is unchanged. Insets show enlargement of leading edges. Bars, 10 µm.