Video 6
RNAi-resistant EBP50-S162,339,340D is unable to form microvilli in siEBP50-treated cells. JEG-3 cells expressing ezrin-GFP (green) and RNAi-resistant TagRFPT-EBP50 PKC site phospho-mimetic mutant (S162,339,340D (red) were treated with siRNA against EBP50. Images were analyzed by time-lapse microscopy. Frames were acquired every 15 s for 7 min, maximum projections of confocal slices for each time point are shown at 5 frames per second.