Video 2.
Actin localization in an epithelial cyst developing in low spreading (high confinement) conditions. MDCK cells stably expressing Life-actin-GFP (gray) were seeded on 700-µm2 collagen I–coated micropatterns to grow cysts and analyzed by time-lapse epifluorescence microscopy with a microscope (AF6000 LX; 40× dry objective). Frames were taken every 10 min for 48 h. After 48 h, DNA was stained with cell-permeable Hoechst at the end of the video recording. Time is given in hours and minutes. Bar, 10 µm.