Video 2.
Tomographic series and model of a vps1Δ cell. Tomographic series and model of a vps1Δ cell at 31,000× (pixel size is 0.764 nm). Dual-axis electron micrographs (Tecnai F30 FEG with SerialEM software) of three 250-nm serial sections from yeast used to generate the tomographic model shown in Fig. 6 are shown in the video. Contour tracings from every 5 nm of membranes (3DMOD) is also shown. The color code is: yellow, multivesicular bodies; purple, tubules; orange, cisterna-shaped endosomes. Bar, 100 nm.