Video 2.
Transformation of blebs into a pseudopod. An aggregation-competent Ax2 cell expressing ABD-RFP (red) as a reporter for F-actin and cAR1-GFP (green) as a marker for the plasma membrane is observed on a glass coverslip under KK2 buffer. Two successive blebs form on the flank of the cell, leaving F-actin scars behind. F-actin rapidly polymerizes (within 1 s) at the bleb membranes to reconstitute the cortex, but polymerization continues, transforming the blebs into a pseudopod. Movie taken by DIC and laser-scanning confocal microscopy (model LSM 710; Carl Zeiss) at 1 frame per second for 50 s.