Video 10.
Effect of FKBP-PIP5K recruitment on the late endosomal/lysosomal localization of GFP-PH-PLCĪ“1. Time-lapse confocal (LSM 780; Carl Zeiss) images of the indicated constructs were acquired every 30 s over 22 min and play 240 times faster than acquisition. Data are from Fig. 6 D. Addition of rapamycin (rapa) after frame 5 is indicated.