Video 4.
Effect of 5 µg/ml Brefeldin A on the localization of PH-FAPP1-GFP and mCherry-P4M (as indicated). Data are time-lapse confocal images acquired with a spinning-disk confocal microscope (Eclipse Ti [Nikon]; CSU-X1[Yokogawa Corporation of America]). The video was acquired at 30 s per frame over 30 min and plays 360 times faster than acquisition. Data are from Fig. 3. Addition of Brefeldin A (BFA) at 10 min is indicated.