Video 9.
Effect of PJ-Sac recruitment on the late endosomal/lysosomal localization of GFP-P4M. Time-lapse confocal (LSM 780; Carl Zeiss) images of the indicated constructs were acquired every 30 s over 10 min and play 240 times faster than acquisition. Data are from Fig. 6 B. Addition of rapamycin (rapa) after frame 5 is indicated.