Table 1.

Phenotypes of new NDC1 alleles

Binding by MYTHaGrowth on 5-FOAb
GenotypePom34Nbp1Mps3POM152pom152Δ
NDC1+++++++++
ndc1-V180G
ndc1-A290E++−/+tsts
ndc1-A527E++−/+++ND
ndc1-A298K+++
ndc1-L294R++−/+
ndc1-E293A+++
ndc1-R306D++++++++
ndc1-V340Q++++
ndc1-L562S++++−/+++
  • ND, not determined.

  • a ++, indicates robust binding; +, indicates binding; −/+, indicates weak binding; −, indicates no binding.

  • b ++, indicates growth at all temperatures; −, indicates no growth at all temperatures; ts, indicates growth at 23°C but not 37°C.