Table 1.

Primers used in this study

PrimersForwardReverse
P[acman] FERM-GalK5′-TCATCCTCTTCATCATCATCTGCATTATCCGACGCAATCGGGGCGGAAAGCCTGTTGACAATTAATCATCGGCA-3′5′-TCTTCGGGATAATCCCGCCGGCCGTTGGCCAGCTCCCGATCGTGGACATCTCAGCACTGTCCTGCTCCTT-3′
P[acman] ΔFERM5′-TGGCCCTGGCCTTCATCATCATCCTCTTCATCATCATCTGCATTATCCGACGGGAGCTGGCCAACGGCCGGCGGGATTATCCCGAAGAGGGCGGATTCCA-3′5′-TGGAATCCGCCCTCTTCGGGATAATCCCGCCGGCCGTTGGCCAGCTCCCGTCGGATAATGCAGATGATGATGAAGAGGATGATGATGAAGGCCAGGGCCA-3′
pUAST-NrgS213L5′-CCGATTTCTACTATGCCTGCTTGGCCACCTCGGTGTTTCGCAG-3′5′-CTGCGAAACACCGAGGTGGCCAAGCAGGCATAGTAGAAATCGG-3′
Check and seq primers
P[acman] ΔFERM check5′-TCCATGTACAGGATCAAGG-3′5′-ACTCTAACCTGTATCGCCATC-3′
P[acman] ΔFERM seq5′-CTTTAACACGGAGAGTGCCAC-3′5′-GATTTTGGGACTTACGGTTGC-3′