Table I

INCENP Concentrates in the Presumptive Cleavage Furrow before Myosin Heavy Chain in Anaphase Cells

Anaphase stageINCENP stainingMyosin stainingChromatid separationFurrowingNumber
μm
ISpindleCytoplasmic<2No 47/717
Earliest(6.5%)
anaphase
IISpindleCytoplasmic2–4No102/717
Early+(14%)
anaphaseEquatorial
cortex
IIISpindleCytoplasmic3–5No215/717
Mid-++(30%)
anaphaseEquatorialEquatorial
cortexcortex
IVSpindleCytoplasmic>4Yes353/717
Late++(49%)
anaphaseFurrowFurrow