Table I

Comparison of Various β1-Integrin Antibody Treatments on Colony Size in 3-Dimensional Culture

DescriptionMean colony sizeRelative size ratio of T4-2/S-1 colonies*
μm
S-1 50 cells   24.5 ± 0.7N/A
Control T4-2 cells  (nonspecific rat IgG's)‡74.3 ± 2.43.03 ± 0.03
Inhibitory β1-antibody-treated  cells, clone AIIB2 crude ascites24.1 ± 1.00.98 ± 0.10
Inhibitory β1-antibody-treated  cells, clone AIIB2 purified IgG128.2 ± 1.11.15 ± 0.04
Inhibitory β1-antibody-treated  cells, clone AIIB2 Fab fragments24.5 ± 1.41.04 ± 0.04
Inhibitory β1-antibody-treated  cells, clone JB1a crude ascites  (alternate inhibitory mAb)32.3 ± 1.41.32 ± 0.06
Activating β1-antibody-treated cells,  clone TS2/16 crude ascites  (stimulatory mAb)61.7 ± 3.32.52 ± 0.13
  • *  The diameter of 200 S-1 acini as observed directly by phase contrast microscopy, were measured after 12 d in 3-D culture using an eyepiece equipped with a micrometer spindle. The same measurements were made on 100–400 T4-2 colonies in the presence of 100 μg antibody ascites protein or 25 μg purified IgG/ml EHS matrix of various β1-integrin antibodies (listed above). The average measurements of each of these groups ± SEM were calculated and the ratio of T4-2/S-1 diameters ± SEM were determined.  

  • Comparable results were also obtained with nonfunction altering antibodies α6- (clone- J1B5) and β1-integrin (clone DF5), which are not shown.