Table I

Yeast Strains Used in This Study

DDY182MAT a ura3-521
DDY1132MAT a/α ura3-52/ura3-521
R757MAT α ura3-52, his4-15, lys92
R2563MAT α ura3-52, his4-15, lys9, Δerg62
DDY186MAT α ura3-52, leu2-3,112,1
DDY321MATahis3-200, leu2-3,112, ura3-52,  Δabp1::LEU21
DDY332MATa, ura3-52, his3-Δ200, Δsla1-Δ1::URA31
DDY544MAT α ura3-52, ade2, leu2-3,112,  Δsla2-Δ1::URA31
DDY771MAT α, his4 ura3-52, Δsac6::URA31
RH144-3DMATa, ura3-52, leu2-3,112, his4, bar1-13
RH266-1DMATa, ura3-52, leu2-3,112, his4, bar1-1,  end3-13
DDY 816MAT α, his3-200, leu2-3,112, ura3-521
DDY 817MAT α, his3-200, leu2-3,112, ura3-52,  Δsrv2/CAP::HIS31
DDY199MATahis3, leu2, ura3, ade2, ade3, can1, sap34
DDY200MATahis3, leu2, ura2, ade3, can1, sap3,  Δtpm1::URA34
YJC093MATarho+, his3-11, leu2-3,112, trp1-1, ura3-15
YJC108MATarho+, his3-11, leu2-3,112, trp1-1,  ura3-1, cap2-Δ1::HIS35
DDY280MATα ura3-62, leu2-3,112, trp1-1, his4-619,  bem1Δ::LEU26
DDY1157MATaura3-52/ura3-52, Δsec8::URA3/  Δsec8::URA3, leu2,3-112::LEU2-Sec8-myc/  leu2-3,112:LEU2-Sec8-myc7
JZ414MAT aura3-52/ura3-52, his3Δ200/  his3Δ200, leu2-Δ1/leu2-Δl lys2-801.  trp1-Δ1/trp1-Δ1 + pBUD6,URA38
M-442MATaura3-52/ura3-52, his3Δ200/  his3Δ200, leu2-Δ1/leu2-Δ1 lys2-801/lys2-801,  trp1-Δ1/trp1-Δ1, gin4Δ::HIS3/gin4Δ::HIS3,  GIN4-GST::URA3/GIN4-GST::URA38
*DDY336MATa, act1-133::HIS3, can1-1, cry11
*DDY337MATa, act1-108::HIS3, can1-1, cry11
*DDY338MATa, act1-101::HIS3, can1-1, cry11
*DDY339MATa, act1-102::HIS3, ade2-101, can1-11
*DDY340MAT α, act1-104::HIS3, can1-11
*DDY341MATa, act1-111::HIS3, ade2-101, cry11
*DDY342MATa, act1-113::HIS3, can1-11
*DDY343MAT α, act1-115::HIS3, ade2-201, can1-11
*DDY344MAT α, act1-116::HIS3, can1-11
*DDY345MATa, act1-117::HIS3, ade2-101, cry11
*DDY346MATa, act1-119::HIS3, ade2-101, cry11
*DDY347MATa, act1-120::HIS3, can1-1, cry11
*DDY348MATa, act1-123::HIS3, ade2-101, can1-1, cry11
*DDY349MAT α, act1-124::HIS31
*DDY350MAT α, act1-125::HIS31
*DDY351MAT α, act1-129::HIS3, can1-11
*DDY352MAT a, act1-132::HIS3, ade2-101, can1-1, cry11
*DDY353MAT α, act1-135::HIS31
*DDY354MATa, ACT1::HIS3, cry11
*DDY355MATa, act1-112::HIS3, ade2-101, ade4, cry11
*DDY356MAT α, act1-105::HIS3, can1-11
*DDY654MAT α, act1-121::HIS3, can1-11
972h- wild-type Schizosaccharomyces pombe9
  • * These strains are a congenic collection of act1 alleles made as described by Wertman et al. (1992). Only alleles that vary between strains are listed above. All strains are his3-Δ200, ura3-52, leu2-3,112, and tub2-201. Sources of strains listed. (1). This lab; (2) R. Gaber (Northwestern University, Evanston, IL); (3) H. Riezman (Biozentrum, University of Basel, Switzerland); (4) A. Bretscher (Cornell University, Ithaca, NY); (5) J. Cooper (Washington University, St. Louis, MO); (6) I. Herskowitz (UCSF, San Francisco, CA); (7) Dan TerBush, Peter Novick (Yale University School of Medicine, New Haven, CT); (8) J. Pringle (University of North Carolina, Chapel Hill, NC); (9) Paul Nurse (ICRF, London, UK).