Table II

Primary Antibodies Used in This Study

AntigenRaised inDilution usedSource
actinRabbit1/401
Cdc42pRabbit1/3002
Bem1pRabbit1/103
Cdc10pRabbit1/103
Cdc11pRabbit1/103
Abp1pRabbit1/2001
cofilinRabbit1/1501
Sla1pRabbit1/7.51
Sla2pRabbit1/501
Bud6p/Aip3pRabbit1/23
Arp2pRabbit1/304
Sec4pMouse1/105
Myc 9E10Mouse1/506
 (for Sec8p)
Smy1pRabbit1/107
Myo2pRabbit1/207
calmodulinRabbit1/508
Bni4pRabbit1/1003
GST (for Gin4p)Rabbit1/201
Spa2pRabbit1/4009
  • Sources: (1) Drubin lab (Drubin et al., 1988; Moon et al., 1993; Ayscough, K., and S. Yang, unpublished observations); (2) D. Johnson (University of Vermont, Burlington, VT; Ziman et al., 1993); (3) J. Pringle (University of North Carolina, Chapel Hill, NC; Ford and Pringle, 1991); (4) B. Winsor (Strasbourg, France; Moreau et al., 1996); (5) P. Novick (Yale University School of Medicine, New Haven, CT; Novick and Brennwald, 1993); (6) Santa Cruz Biotechnology Inc., Santa Cruz, CA; (7) S. Brown (University of Michigan Medical School, Ann Arbor, MI; Lillie and Brown, 1994); (8) M. Stark (Biochemistry Department, University of Dundee, Scotland, UK); (9) M. Snyder (Department of Biology, Yale University, New Haven, CT; Snyder, 1989).