Table I

Yeast Strain Genotype and Construction

StrainGenotypeDerivation
W303Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100
W303αMatα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100
SWY385Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trpl-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup116::URA3 nup116ΔGLFG::TRP1/+Integrative transformation of SWY30  with pSW251(TRP1)
SWY386Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116::URA3 nup116ΔGLFG::TRP1Segregant of tetrad from SWY385
SWY387Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116::URA3 nup116ΔGLFG::TRP1Segregant of tetrad from SWY385
SWY480Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup100::TRP1 nup100ΔGLFG::LEU2/+Integrative transformation of SWY7  with pSW263(LEU2)
SWY545Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup145::LEU2 nup145ΔGLFG::URA3/+Integrative transformation of  SWY203 with pSW280 (URA3)
SWY561Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 kap95::HIS3 pSW271(URA3)Iovine et al., 1995
SWY581Mata ade2-1 ura3-1his-11,15 trp1-1 leu2-3,112 can1-100 nup145::LEU2 nup145ΔGLFG::URA3Segregant of tretrad from SWY545
SWY583Mata ade2-1ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup100::TRP1 nup100ΔGLFG::LEU2Segregant of tetrad from SWY480
SWY585Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup145::LEU2 nup145ΔGLFG::URA3/+ nup100::TRP1 nup100ΔGLFG::  LEU2/+Cross of SWY581 and SWY583
SWY588Matα ade2-1 ura3-1 his3-11,15 tpr1-1 leu2-3,112 can1-100 nup145::LEU2 nup145ΔGLFG::URA3  nup100::TRP1 nup100ΔGLFG::LEU2Segregant of tetrad from SWY585
SWY656Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup145::LEU2 pSW363 (HIS3)Segregant of tetrad from SWY556
SWY958Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116::HIS3 pSW461(URA3)Transformation of SWY27 with  pSW461 (URA3)
SWY960Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup116::HIS3/nup116::HIS3 pSW461(URA3)Cross of SWY958 and SWY29  carrying pRS314 (TRP1)
SWY1312Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 kap95::HIS3 pSW503(LEU2)Transformation of SWY561 with  pSW503(LEU2) and selected on  5-FOA
SWY1313Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 kap95::HIS3 pSW509(LEU2)Transformation of SWY561 with  pSW509(LEU2) and selected on  5-FOA
SWY1379Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup100::TRP1/nup100::HIS3Cross of SWY9 and SWY238
SWY1381Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup100::TRP1/nup100::HIS3 ProteinA-NUP100::LEU2Integrative transformation of  SWY1379 with pSW645 (LEU2)
SWY1400Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup116::URA3 nup116ΔGLFG::TRP1/+ nup100::TRP1 nup100ΔGLFG::  LEU2/+Cross of SWY387 and SWY583
SWY1401Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup100::TRP1 nup100ΔGLFG::LEU2  pSW132(URA3)Transformation of SWY583 with  pSW132(URA3)
SWY1402Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116ΔGLFG5-FOA selected SWY386
SWY1404Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup116ΔGLFG/+ nup100::TRP1 nup100ΔGLFG::LEU2/+5-FOA selected SWY1400
SWY1405Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup116ΔGLFG/+ nup100::TRP1 nup100ΔGLFG::LEU2/+ pSW131(URA3)Transformation of SWY1404 with  pSW131(URA3)
SWY1406Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116ΔGLFG nup100::TRP1  nup100ΔGLFG::LEU2 pSW131(URA3)Segregant of tetrad from SWY1405
SWY1407Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116ΔGLFG pSW131(URA3)Transformation of SWY1402 with  pSW131(URA3)
SWY1428Mata/Matα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112  can1-100/can1-100 nup116ΔGLFG/+ nup145::LEU2/+ pSW131 (URA3) pSW363 (HIS3)Cross of SWY656 and SWY1407
SWY1429Matα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116ΔGLFG nup145::LEU2 pSW131  (URA3) pSW363 (HIS3)Segregant of tetrad from SWY1428
SWY1430Mata ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 nup116ΔGLFG nup145::LEU2 pSW363  (HIS3)Segregant of tetrad from SWY1428  after 5-FOA selection
  • The following strains have been previously described: SWY7, SWY9 in Wente and Blobel, 1994; SWY27, SWY29 in Wente and Blobel, 1993; SWY30 in Wente et al., 1992; SWY491 and SWY561 in Iovine et al., 1995; SWY203, SWY556, and SWY656 in Emtage et al., 1997; SWY1017 in Murphy et al., 1996. All plasmids mentioned above are described in Table II.