Table I

CD44v Expression on Lag+ Epidermal and Dermal Langerhans Cells after Different Culture Intervals of Full-Thickness Skin Explants

EpidermisDermis
Culture period*Number of Lag+‡CD44v5+‡CD44v6+‡CD44v9+*Number of Lag+‡CD44v5+‡CD44v6+‡CD44v9+
     hcells/mm2%%%cells/mm2%%%
 0985.7     04.2 7.8 30.6 10010080.0
 2927.40.92.8 6.4 67.3 95.5 91.683.4
 6927.401.3 6.5 67.3 94.5 94.188.9
12768.42.75.1 7.2 84.6 88.1 89.083.3
24756.103.7 5.6 84.6 88.910075.0
48675.00.75.910.7 76.5 92.3 96.391.0
72609.20.72.9 7.1102.6 87.510091.0
  • *  Full-thickness skin organ culture and immunohistochemistry was performed as described in Fig. 3.  

  •  CD44v positive and negative Lag+ cells were counted in 10 high power fields and the percentage of double-positive cells was calculated. The data represent the mean of four independent experiments, the SD being below 10%.